Rat CD300LF Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80412-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD300LF cDNA Clone Datasheet

CD300LF cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001025111.1
cDNA Size:	951
Gene Synonym:	CD300f; RGD1309733; Cd300lf
Source:	Rat

CD300LF cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD300 molecule-like family member f DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 658-705 deletion mutation.

CD300LF cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD300LF cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD300LF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATTTGT CATTGCTGGC TCTCTTTCTC TTCTGGATCT CAGGCTGCTT CACTGCTCAG
61       GATCCAGTCA CAGGTCCAGA GGAGGTGAGC GGTTACGAGC AGGGCTCCCT GACAGTGTGG
121      TGTCGATATG GCTCATGGTG GAAGGATTAC AGCAAGTACT GGTGCCGAGG ACCTAAGAGG
181      AGTTCCTGTG AGATTCGAGT TGAAACCGAT GCATCAGAGC GCTTGGTGAA GGAGAACCAT
241      GTGTCCATCA GGGACGACCA GACAAACTTC ACCTTCACAG TGACCATGGA GGACCTGAGG
301      ATGAGCGATG CTGGCATTTA CTGGTGTGGA ATTACAAAGG CTGGATATGA TCACATGTTT
361      AAAGTTCATG TGAGCATTAA CCCAGTGCCA ACAACGCCCA CCACCACCTC CACCACCACC
421      ATCTTCACAG TGACAACCAC TGTAAAAGAG ACCAGCACGC TTTCAACGCA GACCAGCCAC
481      TACTCTGATA ACAGGTATGA CAGTGGTGGT GTCGGCGACG GTAATGGGTT TCTGGATCTC
541      AGCGTGCTCC TCCCAGTCAT CTCTGCAGCC CTGTTGCTTC TCTTGCTGGT GGTCTCACTA
601      ATTGCTTGGA GGATGGTGAG GAGACAGAAG AAAGCTGCTG GACCACCCTC AGGGCAGCCT
661      CTGGAGGATG ATCTCTGTTA TGCAAACCTG TCACTGCAGC AGCCCAGAAC CTCCCCTTTG
721      AAAAAGGGCT CCTCCATGTC CTCCTCTGGC AAGGACCACC AAGAGGAAGT GGAATACGTC
781      ACCATGGCTC CCTTCCCTAG GGAGGAGATT TCATACGCTG CTCTGTCTTT GGCGAGCTTG
841      GGTCAGGAAC CTACTTACAG CAACACTGCC TGCCTTGTCA CCCATGGTCC CAGGACAAAC
901      CTTGGAGAGG AGACCACAGA GTACAGCAGC ATCAGGAGGC CCATGCCTTG A

Quality Control:cDNA sequencing results is available upon request.