Rat CD300E Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80411-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD300E cDNA Clone Datasheet

CD300E cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001166577.1
cDNA Size:	615
Gene Synonym:	Cd300e
Source:	Rat

CD300E cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus CD300e molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CD300E cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD300E cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD300E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGCTGT CCATAGGTCT GTTCCTTCTC TGCTTCCAAG GTTGTTTATC TCTGACGGGC
61       CCTGGCTCTG TGTCTGGCTA TGTAGGAGGC TCTCTCCGTG TGCAGTGTCA ATATGCACAA
121      TCATATAAGG GCTACACGAA ATACTGGTGC CGAGGACCGC ATGACAAAGG GTGTAAAAGT
181      ATTGTAGAAA CCGATGGAAG TGAGAGAGAA AAGAAGAATG GCCGAGTGTC CATCAGAGAC
241      CATGCTGGGA ACTCCACCAT CACAGTGACC ATGGAGAACC TCAGTGAAGA CGATGCTGGC
301      TCTTACTGGT GCAAGATTCA GACGGTTTTT ATCTGGGATT TTTTGTCACG TGACCCATCG
361      GTCCGTGTAA AGGTACATGT TTTCCCAGCC ACAACTCCCA TGGAGACCAC ACTCCCAGCC
421      ATAACTCCTT TCCTCCCACT AGTGGATCCT GGACAGAACC TGAGGATTCG TTCTAGCACA
481      ATGCTCATCT TCCAACTATG GTCTCTGCTC AGCAGCGTCC ACTTCCAGGT CCTGGTCTTC
541      CTGAAACTGC CTCTGTTTCT GAGCATGCTC TGTGCTATCT TCTGGGTGAG CAGGAAGATT
601      CCTGAGGGAA TGTAG

Quality Control:cDNA sequencing results is available upon request.