Mouse CD300E Gene cDNA Clone / ORF Clone

CD300E cDNA Clone Datasheet

CD300E cDNA Clone Product Information

• Gene Bank Ref.ID: NM_172050.2
• cDNA Size: 591
• Gene Synonym: CLM2; TREM5; Cd300le; BC034097; MGC31495; MGC49720; Cd300e
• Source: Mouse

CD300E cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus CD300e antigen DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD300E cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD300E cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD300E cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCTAT GTGCAGGTCT GCTCCTTCTC TGCTTCCAAG GTTGTTTGTC TCTGACGGGC
61       CCTGGCTCTG TGTCTGGCTA CGTAGGAGGC TCTCTCCGTG TGCAGTGTCA ATATAGTCCA
121      TCATATAAGG GCTATATGAA ATACTGGTGC CGAGGACCGC ATGACACGAC ATGTAAAACT
181      ATTGTAGAAA CCGACGGAAG TGAGAAAGAA AAGAGGAGTG GCCCGGTGTC CATCAGAGAC
241      CATGCTTCGA ACTCCACCAT CACAGTGATC ATGGAGGACC TCAGCGAAGA CAATGCTGGG
301      TCTTACTGGT GCAAGATTCA GACTTCCTTT ATCTGGGATT CGTGGTCACG TGATCCATCG
361      GTCAGTGTAA GGGTGAATGT TTTTCCAGCC ACAACTCCTA CACTCCCAGC CACAACTGCT
421      ATCCTCCCAC TAGTGAATTC TGGGCAGAAC CTGAGGATTA GTACTAATGT GATGTTCATC
481      TTCCAACTGT GGTCCCTGCT CAGCAGCATC CAGTTCCAGG TCCTGGTCTT CCTGAAGCTG
541      CCTCTGTTTC TGAGCATGCT CTGTGCTATC TTCTGGGTGA ACAGACTTTA G

 

Quality Control:cDNA sequencing results is available upon request.