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CD300C Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for CD300C Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.00245 ng/well in ELISA|
CD300C Antibody Product Information
Recombinant Human CD300C protein (Catalog#11832-H08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human CD300C (rHuman CD300C; Catalog#11832-H08H; NP_006669.1; Met 1-Arg 183). CD300C specific IgG was purified by Human CD300C affinity chromatography.
CD300C Antibody Usage Guide
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD300C in WB. Using a DAB detection system, the detection limit for Human CD300C is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD300C. The detection limit for Human CD300C is approximately 0.00245 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD300C Antibody Related Products & Topics
CD300C Antibody Background
CMRF35-like molecule 6, also known as CD300 antigen-like family member C, Immunoglobulin superfamily member 16, CMRF35-A1, CD300C and CMRF35, is a single-pass type I membrane protein which belongs to theCD300 family. CD300c / CMRF-35 contains oneIg-like V-type (immunoglobulin-like) domain. CD300c / CMRF-35 is present on the surface of monocytes, neutrophils, a proportion of peripheral blood T-lymphocytes and B-lymphocytes and lymphocytic cell lines. The CD300c (CMRF-35A) and CD300a (CMRF-35H) molecules are leukocyte surface proteins that are part of a larger family of immunoregulatory molecules encoded by a gene complex on human chromosome 17. The CD300a protein contains four ITIMs and is highly homologous to CD300c. CD300a/c RNA and surface expression were downregulated after stimulation of plasmacytoid dendritic cells (pDCs) with TLR7 and TLR9 ligands. Exogenous interferon (IFN)-alpha down-regulated CD300a/c expression, whereas neutralizing IFN-alpha abolished TLR ligand-induced CD300a/c down-regulation. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from pDCs. CD300a and CD300c are indistinguishable on the surface of NK cells.
- Jackson D.G., et al., 1992, Eur. J. Immunol. 22:1157-1163.
- Speckman R.A., et al., 2003, Hum. Genet. 112: 34-41.
- Clark,G.J. et al., 2007, J Leukoc Biol82 (5): 1126-35.
- Ju,X. et al., 2008, Blood. 112 (4): 1184-94.
- Lankry,D. et al., 2010, J Immunol. 185 (5): 2877-86.
CD300C related areas, pathways, and other information