CD300C Antibody (Antigen Affinity Purified)

CD300C Antibody ( Antigen Affinity Purified ) Datasheet

CD300C Antibody Product Information

• Immunogen: Recombinant Human CD300C protein (Catalog#11832-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD300C (rHuman CD300C; Catalog#11832-H08H; NP_006669.1; Met 1-Arg 183). CD300C specific IgG was purified by Human CD300C affinity chromatography.

CD300C Antibody Usage Guide

• Specificity: Human CD300C
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD300C in WB. Using a DAB detection system, the detection limit for Human CD300C is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD300C. The detection limit for Human CD300C is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD300C Antibody Background

CMRF35-like molecule 6, also known as CD300 antigen-like family member C, Immunoglobulin superfamily member 16, CMRF35-A1, CD300C and CMRF35, is a single-pass type I membrane protein which belongs to theCD300 family. CD300c / CMRF-35 contains oneIg-like V-type (immunoglobulin-like) domain. CD300c / CMRF-35 is present on the surface of monocytes, neutrophils, a proportion of peripheral blood T-lymphocytes and B-lymphocytes and lymphocytic cell lines. The CD300c (CMRF-35A) and CD300a (CMRF-35H) molecules are leukocyte surface proteins that are part of a larger family of immunoregulatory molecules encoded by a gene complex on human chromosome 17. The CD300a protein contains four ITIMs and is highly homologous to CD300c. CD300a/c RNA and surface expression were downregulated after stimulation of plasmacytoid dendritic cells (pDCs) with TLR7 and TLR9 ligands. Exogenous interferon (IFN)-alpha down-regulated CD300a/c expression, whereas neutralizing IFN-alpha abolished TLR ligand-induced CD300a/c down-regulation. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from pDCs. CD300a and CD300c are indistinguishable on the surface of NK cells.

References

1. Jackson D.G., et al., 1992, Eur. J. Immunol. 22:1157-1163.
2. Speckman R.A., et al., 2003, Hum. Genet. 112: 34-41.
3. Clark,G.J. et al., 2007, J Leukoc Biol82 (5): 1126-35.
4. Ju,X. et al., 2008, Blood. 112 (4): 1184-94.
5. Lankry,D. et al., 2010, J Immunol. 185 (5): 2877-86.