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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CD300A ORF mammalian expression plasmid, C-GFPSpark tag||RG80410-ACG|
|Rat CD300A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80410-ACR|
|Rat CD300A ORF mammalian expression plasmid, C-Flag tag||RG80410-CF|
|Rat CD300A ORF mammalian expression plasmid, C-His tag||RG80410-CH|
|Rat CD300A ORF mammalian expression plasmid, C-Myc tag||RG80410-CM|
|Rat CD300A ORF mammalian expression plasmid, C-HA tag||RG80410-CY|
|Rat CD300A ORF mammalian expression plasmid, N-Flag tag||RG80410-NF|
|Rat CD300A ORF mammalian expression plasmid, N-His tag||RG80410-NH|
|Rat CD300A ORF mammalian expression plasmid, N-Myc tag||RG80410-NM|
|Rat CD300A ORF mammalian expression plasmid, N-HA tag||RG80410-NY|
|Rat CD300A natural ORF mammalian expression plasmid||RG80410-UT|
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Mouse CMRF35-like molecule 8, also known as CD300 antigen-like family member A, CMRF35-H9, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor, CD300a and CMRF35H, is a single-pass type I membrane protein which belongs to the CD300 family. The CD300 family of myeloid immunoglobulin receptors includes activating ( CD300b, CD300e ) and inhibitory members ( CD300a, CD300f ), as well as molecules presenting a negative charge within their transmembrane domain ( CD300c, CD300d ). CD300A / IGSF12 is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.It contains one Ig-like V-type ( immunoglobulin-like ) domain. CD300A / IGSF12 is an inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. CD300c is a functional immune receptor able to deliver activating signals upon ligation in RBL-2H3 mast cells. CD300c signaling is partially mediated by a direct association with the immune receptor tyrosine-based activation motif-bearing adaptor FcεRγ. CD300a and CD300c play an important role in the cross-regulation of TNF-alpha and IFN-alpha secretion from plasmacytoid dendritic cells (pDCs).