Ferret CD3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
FG60005-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD3 cDNA Clone Datasheet

CD3 cDNA Clone Product Information

Gene Bank Ref.ID:	EF492054.1
cDNA Size:	561
Gene Synonym:	CD3
Source:	Ferret

CD3 cDNA Clone Description

cDNA Description:	ORF Clone of Ferret CD3 epsilon antigen DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CD3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCCGG GGAACCTCTG GAGAATTCTG GGACTCTGTC TCTTATTAGT TGGAGCTTGG
61       GGGCAGGAAG AAGGTTATAA AGTCTCCATC TCTGGAACTA TGGTAGTGCT GACATGCCCT
121      GAGGAGGTTG ATACCAACAG TATAACATGG GAAAAAAATG GTGAAGCAAT AAAAGGAGCT
181      AATGAAAGGC AGTACACTAT GCATACATTT TCAGAAGTGG AAGACAGTGG TTCTTATACC
241      TGCTTTTCAG ACAAATTGAA GAAGAACTCT CTCTACCTGA AAGCAAGAGT GTGTAAGAAC
301      TGCATAGAGG TGAGTCCGAT GGCGGTGGCT GCAATCCTCG TCACTGACAT CTGCATTACT
361      CTGGGCTTGC TTCTGCTGGT CTATTACTGG AGCAAGAATA GGAAGGCCAA TGCCACAACT
421      GTGATGAGAG CAAAAGGCGC TGGCGGCAGG ACTAGGGGAC AAAACAAGGA GAAGCCACCA
481      CCTGTTCCCA ATCCGGACTA TGAGCCCATC CGGAAAGGCC AGCAGGACCT GTATTCCGGC
541      CTGAATCAGA GAGGCATCTG A

Quality Control:cDNA sequencing results is available upon request.