Human CD2AP Gene cDNA Clone / ORF Clone

CD2AP cDNA Clone Datasheet

CD2AP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012120.2
• cDNA Size: 1920
• Gene Synonym: CMS; DKFZp586H0519; CD2AP
• Source: Human

CD2AP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens CD2-associated protein DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1204 C/T not causing the amino acid variation.

CD2AP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD2AP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD2AP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTGACT ATATTGTGGA GTATGACTAT GATGCTGTAC ATGATGATGA ATTAACTATT
61       CGAGTTGGAG AAATCATCAG GAATGTGAAA AAGCTACAGG AGGAAGGGTG GCTGGAAGGA
121      GAACTAAATG GGAGAAGAGG AATGTTCCCT GACAATTTCG TTAAGGAAAT TAAAAGAGAG
181      ACGGAATTCA AGGATGACAG TTTGCCCATC AAACGGGAAA GGCATGGGAA TGTAGCAAGT
241      CTTGTACAAC GAATAAGCAC CTATGGACTT CCAGCTGGAG GAATTCAGCC ACATCCACAA
301      ACCAAAAACA TTAAGAAGAA GACCAAGAAG CGTCAGTGTA AAGTTCTTTT TGAGTACATT
361      CCACAAAATG AGGATGAACT GGAGCTGAAA GTGGGAGATA TTATTGATAT TAATGAAGAG
421      GTAGAAGAAG GCTGGTGGAG TGGAACCCTG AATAACAAGT TGGGACTGTT TCCCTCAAAT
481      TTTGTGAAAG AATTAGAGGT AACAGATGAT GGTGAAACTC ATGAAGCCCA GGACGATTCA
541      GAAACTGTTT TGGCTGGGCC TACTTCACCT ATACCTTCTC TGGGAAATGT GAGTGAAACT
601      GCATCTGGAT CAGTTACACA GCCAAAGAAA ATTCGAGGAA TTGGATTTGG AGACATTTTT
661      AAAGAAGGCT CTGTGAAACT TCGGACAAGA ACATCCAGTA GTGAAACAGA AGAGAAAAAA
721      CCAGAAAAGC CCTTAATCCT ACAGTCACTG GGACCCAAAA CTCAGAGTGT GGAGATAACA
781      AAAACAGATA CCGAAGGTAA AATTAAAGCT AAAGAATATT GTAGAACATT ATTTGCCTAT
841      GAAGGTACTA ATGAAGATGA ACTTACTTTT AAAGAGGGGG AGATAATCCA TTTGATAAGT
901      AAGGAGACTG GAGAAGCTGG CTGGTGGAGG GGCGAACTTA ATGGTAAAGA AGGAGTATTT
961      CCAGACAATT TTGCTGTCCA GATAAATGAA CTTGATAAAG ACTTTCCAAA ACCAAAGAAA
1021     CCACCACCTC CTGCTAAGGC TCCAGCTCCA AAGCCTGAAC TGATAGCTGC AGAGAAGAAA
1081     TATTTTTCTT TAAAGCCTGA AGAAAAGGAT GAAAAATCAA CACTGGAACA GAAACCTTCT
1141     AAACCAGCAG CTCCACAAGT CCCACCCAAG AAACCTACTC CACCTACCAA AGCCAGTAAT
1201     TTACTGAGAT CTTCTGGAAC AGTGTACCCA AAGCGACCTG AAAAACCAGT TCCTCCACCA
1261     CCTCCTATAG CCAAGATTAA TGGGGAAGTT TCTAGCATTT CATCAAAATT TGAAACTGAG
1321     CCAGTATCAA AACTAAAGCT AGATTCTGAA CAGCTGCCCC TTAGACCAAA ATCAGTAGAC
1381     TTTGATTCAC TTACAGTAAG GACCTCCAAA GAAACAGATG TTGTAAATTT TGATGACATA
1441     GCTTCCTCAG AAAACTTGCT TCATCTCACT GCAAATAGAC CAAAGATGCC TGGAAGAAGG
1501     TTGCCGGGCC GTTTCAATGG TGGACATTCT CCAACTCACA GCCCCGAAAA AATCTTGAAG
1561     TTACCAAAAG AAGAAGACAG TGCCAACCTG AAGCCATCTG AATTAAAAAA AGATACATGC
1621     TACTCTCCAA AGCCATCTGT GTACCTTTCA ACACCTTCCA GTGCTTCTAA AGCAAATACA
1681     ACTGCTTTCC TGACTCCATT AGAAATCAAA GCTAAAGTGG AAACAGATGA TGTGAAAAAA
1741     AATTCCCTGG ATGAACTTAG AGCCCAGATT ATTGAATTGT TGTGCATTGT AGAAGCACTG
1801     AAAAAGGATC ACGGGAAAGA ACTGGAAAAA CTGCGAAAAG ATTTGGAAGA AGAGAAGACA
1861     ATGAGAAGTA ATCTAGAGAT GGAAATAGAG AAGCTGAAAA AAGCTGTCCT GTCTTCTTGA

 

Quality Control:cDNA sequencing results is available upon request.