Rat CD28 Gene cDNA Clone / ORF Clone

CD28 cDNA Clone Datasheet

CD28 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013121.1
• cDNA Size: 657
• Gene Synonym: CD28RNA; Cd28
• Source: Rat

CD28 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus Cd28 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 310 G/C, 457 C/G resulting in the amino acid Asp substitution by His, Pro substitution by Ala and 309 C/G，603 A/C not causing the amino acid variation.

CD28 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD28 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD28 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACACTCA GACTGCTATT CTTGGCTCTC AGCTTCTTCT CAGTTCAAGT AACAGAAAAC
61       AAGATTTTGG TGAAGCAGTC CCCGCTGCTT GTGGTAGATA ACAATGAGGT CAGCCTCAGC
121      TGCAGGTATT CCTACAACCT TCTTGCAAAG GAGTTCCGGG CATCCCTTTA CAAGGGCGTG
181      AACAGTGACG TGGAAGTCTG TGTCGGGAAT GGGAATTTTA CCTATCAGCC CCAGTTCCGC
241      CCAAACGTGG GGTTCAACTG TGATGGGAAT TTCGACAATG AAACAGTGAC GTTCCGTCTC
301      TGGAATCTGC ATGTTAATCA CACAGATATT TACTTCTGCA AAATCGAGGT CATGTATCCT
361      CCGCCATACC TGGACAACGA GAAGAGCAAC GGAACTATTA TTCACATAAA AGAGAAACAT
421      CTTTGTCATG CTCAGACGTC TCCTAAGCTG TTTTGGGCAC TGGTCGTGGT TGCTGGAGTC
481      CTGCTGTGTT ATGGCTTGCT AGTGACAGTG ACTCTTTGTA TTATCTGGAC AAATAGCAGA
541      AGGAACAGGC TCCTTCAAAG TGACTACATG AACATGACAC CGCGGAGACT CGGGCCCACG
601      CGCAAGCATT ACCAACCCTA TGCCCCAGCG AGAGACTTTG CAGCGTACCG CCCCTGA

 

Quality Control:cDNA sequencing results is available upon request.