Anti-CD28 Antibody

Cat: 11524-MM06
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Anti-CD28 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD28 Antibody
Validated applications
ELISA,ELISA(Cap)
Species reactivity
Reacts with: Human
Specificity
Human CD28
No cross-reactivity in ELISA with Mouse CD28 Human CTLA4 Mouse CTLA4 Human B7-1 Human B7-2 Mouse B7-2 Human β-NGF Human cell lysate (293 cell line)
Immunogen
Recombinant Human CD28 protein (Catalog#11524-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD28 (rh CD28; Catalog#11524-H08H; P10747-1; Met 1-Pro 152). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9H6E2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD28 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human CD28 / TP44 in ELISA pair set (Catalog: # SEK11524). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 11524-MM04).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD28 Antibody Alternative Names
Anti-Tp44 Antibody
CD28 Background Information

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD28 molecule
References
  • Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
  • Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
  • Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD28 Antibody Mouse Monoclonal ELISA 80302-MM04
    Anti-CD28 Antibody Rabbit Polyclonal ELISA 80302-T16
    Anti-CD28 Antibody Rabbit Polyclonal WB,ELISA 50103-T48
    Anti-CD28 Antibody Rabbit Polyclonal ELISA 50103-RP01
    Anti-CD28 Antibody Rabbit Polyclonal ELISA,IHC-P 50103-RP02
    Anti-CD28 Antibody Rabbit Monoclonal ELISA,FCM 11524-R007
    Anti-CD28 Antibody (FITC) Rabbit Monoclonal FCM 11524-R007-F
    Anti-CD28 Antibody FCM,Func/A 11524-H001
    Anti-CD28 Antibody (PerCP) Rabbit Monoclonal FCM 11524-R007-C
    Anti-CD28 Antibody Rabbit Polyclonal ELISA 11524-RP01
    Anti-CD28 Antibody Rabbit Polyclonal ELISA 11524-RP02
    Anti-CD28 Antibody Mouse Monoclonal ELISA(Det) 11524-MM04
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