CD28 Antibody (Antigen Affinity Purified)

CD28 Antibody ( Antigen Affinity Purified ) Datasheet

CD28 Antibody Product Information

• Immunogen: Recombinant Human CD28 protein (Catalog#11524-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD28 (rh CD28; Catalog#11524-H08H; P10747-1; Met 1-Pro 152). CD28 specific IgG was purified by Human CD28 affinity chromatography.

CD28 Antibody Usage Guide

• Specificity: Human CD28
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD28 in WB. Using a DAB detection system, the detection limit for Human CD28 is approximately 4 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD28. The detection limit for Human CD28 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD28 Antibody Background

T-cell-specific surface glycoprotein CD28, also known as TP44 and CD28, is a single-pass type I membrane protein which contains oneIg-like V-type (immunoglobulin-like) domain. CD28 / TP44 is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD28 / TP44 is expressed in T-cells and plasma cells, but not in less mature B-cells. CD28 / TP44 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. CD28 / TP44 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. CD28 / TP44 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

References

1. Linsley, P. S. et al., 1993, Annu Rev Immunol11:191-212.
2. Edmead, C. E. et al., 1997, Int J Biochem Cell Biol.29 (8-9):1053-1057.
3. Woerly, G. et al., 2002, J.Leukoc. Biol. 72 (4): 769-79.