|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD27 antigen-like DNA.|
|KpnI + XhoI|
|FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG|
|Identical with XM_001115263.2 [ Macaca mulatta (Rhesus monkey) ]: 180 C/T not causing the amino acid variation. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone)||CG90049-G|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90049-G-H|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90049-G-M|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90049-G-N|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90049-G-Y|
|Product name||Product name|
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.