+86-400-890-9989
Product Catalog
Research Topics
Your Position: Home
> Gene Products > Other Species > Cynomolgus monkey CD27 ORF Expression Clone, FLAG-tagged Cynomolgus monkey CD27 ORF Expression Clone, FLAG-tagged
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| CG90049-G-F |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
CD27 cDNA Clone Datasheet
CD27 cDNA Clone Product Information
|
cDNA Size: |
810 |
| Gene Synonym: |
CD27 |
| Source: | Cynomolgus monkey |
CD27 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD27 antigen-like DNA. |
| Vector: | |
| FLAG Tag Sequence: | GATTACAAGGATGACGACGATAAG |
| Sequence Description: |
Identical with the Gene Bank Ref. ID sequence except for the point mutation 180 C/T not causing the amino acid variation. |
CD27 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
CD27 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.
CD27 cDNA Clone Sequence and Quality Control
Sequence:
which has no influence on protein expression.
2. FLAG Tag
1 ATGGCACGGC CACATCCCTG GTGGCTGTGC TTTCTGGGGA CCCTGGTGGG GCTCTCAGCT 61 ACTCCAGCCC CCAAGAGCTG CCCAGAGAGG CACTACTGGG CTCAGGGAAA ACTGTGCTGC 121 CAGATGTGTG AGCCAGGAAC ATTCCTTGTG AAGGACTGTG ACCAGCACAG AAAGGCTGCT 181 CAGTGTCATC CTTGCATACC AGGGGTCTCC TTCTCTCCAG ACCACCACAC CCGGCCTCAC 241 TGTGAGAGCT GTCGGCACTG TAACTCTGGT CTTCTCATTC GCAACTGCAC CATCACTGCC 301 AACGCTGTGT GTGCCTGTCG CAATGGCTGG CAGTGCAGGG ACAAGGAGTG TACTGAGTGT 361 GATCCTCCTC CAAACCCTTC GCTGACCACT TGGCCATCTC AGGCCCTGGG CCCACACCCT 421 CAGCCCACCC ACTTACCTTA TGTCAATGAG ATGCTGGAGG CCAGAACAGC AGGGCACATG 481 CAGACTCTGG CTGACTTCAG GCACCTGCCT GCCCGGACTC TCTCTACCCA CTGGCCACCC 541 CAAAGATCCC TGTGCAGCTC AGATTTTATT CGTATCCTTG TGATCTTCTC CGGAATGTTT 601 CTTGTTTTCA CCCTGGCCGG AACCCTGTTC CTCCATCAAC AAAGGAAATA TAGATCAAAC 661 AAAGGAGAAA GTCCCATGGA GCCTGCAGAA CCTTGTCCTT ACAGCTGCCC CAGGGAGGAG 721 GAAGGCAGCA CCATCCCCAT CCAAGAGGAT TACCGAAAAC CGGAGCCTGC CTCCTCCCCG 781 GCT1GATTACA AGGATGACGA CGATAAG2TAA
1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag
Quality Control:cDNA sequencing results is available upon request.
