|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD27 antigen-like DNA.|
|Identical with XM_001106493.2 [ Macaca mulatta (Rhesus monkey) ]: 180 C/T not causing the amino acid variation. Please check the sequence information before order.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||CG90049-G-F|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||CG90049-G-H|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||CG90049-G-M|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||CG90049-G-N|
|Cynomolgus monkey CD27 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||CG90049-G-Y|
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CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.