CD27 / TNFRSF7 Protein (Cytokine)

CD27 molecule Protein Datasheet

CD27 / TNFRSF7 Protein Product Information

• Synonym: CD27, TNFRSF7
• Protein Construction: A DNA sequence encoding the human CD27 (P26842)(Met1-Ile192) was expressed, fused with the Fc region of rabbit IgG at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CD27 / TNFRSF7 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized human CD27-his (Cat:10039-H31H) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (Cat:10780-H01H), The EC₅₀ of biotinylated human CD70-Fc (Cat:10780-H01H) is 95-150 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 20
• Molecular Mass: The recombinant human CD27/rFc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.4 kDa. The apparent molecular mass of the protein is approximately 63 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD27 / TNFRSF7 protein

CD27 / TNFRSF7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD27 / TNFRSF7 Protein Description

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

References

1. Borst J. et al., 2005, Curr Opin Immunol. 17: 275-81.
2. Kobata T. et al., 1995, Proc Natl Acad Sci. 92: 11249-53.
3. Jacquot S. 2000, Immunol Res. 21: 23-30.
4. Arens R. et al., 2004, J Immunol. 173: 3901-8.
5. Akiba H. et al., 1998, J Biol Chem. 273: 13353-8.
6. Fortin A. et al., 2004, J Biol Chem. 279: 28706-14.