CD27 / TNFRSF7 Antibody ( FITC )

CD27 / TNFRSF7 Antibody Datasheet

CD27 / TNFRSF7 Antibody Product Information

• Immunogen: Recombinant Mouse CD27 protein (Catalog#50110-M08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 012
• Ig Type: Rabbit IgG
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 / TNFRSF7 (rM CD27 / TNFRSF7; Catalog#50110-M08H; NP_001028298.1; Met 1-Arg 182) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD27 / TNFRSF7 Antibody Usage Guide

• Specificity: Mouse CD27 / TNFRSF7
• Has cross-reactivity in ELISA with
  

  Human CD27
  Human BAFF

  No cross-reactivity in ELISA with
  

  Human TNFRSF8
  Human CD40
  Human DR6
  Human TNFRSF10D
  Mouse HVEM
  Mouse FAS
  Mouse TNFRSF10b
  Human 4-1BB
  Human TNFRI
  Human TNFRII
  Human RELT

• Flow Cytometry: Expression of CD27 on mouse splenocytes.
  BABL/c splenocytes were stained simultaneously with FITC-conjugated mAb R012 and PE-conjugated anti-mouse CD3 mAb (BD Pharmingen™Cat. No. 553064).
  
  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD27 / TNFRSF7 Antibody Background

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

References

1. Borst. J. et al., 2005, Curr. Opin. Immunol. 17: 275-281.
2. Kobata. T. et al., 1995, Proc. Natl. Acad. Sci. 92: 11249-53.
3. Jacquot, S., 2000, Immunol Res. 21:23-30.
4. Arens, R. et al., 2004, J. Immunol. 173: 3901-3908.
5. Akiba, H. et al., 1998, J. Biol. Chem. 273: 13353-13358.
6. Fortin, A. et al., 2004, J. Biol. Chem. 279: 28706-28714.