Mouse CD27 / TNFRSF7 Protein (His Tag) (Cytokine)

Mouse CD27 / TNFRSF7 Protein

CD27 / TNFRSF7 Protein Product Information

• Synonym: S152, Tnfrsf7, Tp55
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Arg 182 ) of mouse CD27 ( NP_001028298.1 ) was fused with a polyhistidine tag at the C-terminus
• Source: Mouse
• Expression Host: Human Cells

CD27 / TNFRSF7 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized recombinant mouse CD27 at 2 μg/mL ( 100 μl/well ) can bind human CD70 with a linear range of 78 - 1250 pg/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Thr 21
• Molecular Mass: The recombinant mouse CD27 consists of 173 amino acids after removal of the signal peptide and has a predicted molecular mass of 19.6 kDa. In SDS-PAGE under reducing conditions, rmCD27 migrates as an approximately 35 kDa band due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: TNFRSF7 protein

CD27 / TNFRSF7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD27 / TNFRSF7 Protein Description

Mouse CD27, also known as TNFRSF7, is a member of the tumor necrosis factor receptor (TNFR) superfamily characterized by the presence of a Cys-knot motif in the extracellular region, which occurs three times in CD27. CD27 is a type I  transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, and has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis. CD27 has been defined as a T and B cell co-stimulatory molecule, and its activity is governed by the transient availability of its TNF-like ligand CD70 on lymphocytes and dendritic cells. In man and mouse, CD27 is restricted to lymphoid cells and present on the great majority of naive CD4+ and CD8 + T cells. CD27 may contribute to normal murine T cell development by synergizing with the pre-TCR-mediated signal, since the CD27/CD70 receptor-ligand pair cooperates with the TCR in the regulation of the peripheral T cell response. Murine CD27 shows an identity of 65% in aa sequence compared with human CD27.

References

1. K. V.S. Prasad, et al., 1997, Proc. Natl. Acad. Sci. USA. 94: 6346-51
2. Loes A.G, 2003, European Journal of Immunology, 23: 943-50.
3. Anne Wiesmann, et al., 2000, Immunity. 12: 193-9.
4. Jannie Borst, et al., 2005, Current Opinion in Immunology,17: 275-81. 
5. Loes A. G, et al., 1996, J. Exp. Med. 184: 675-85.
6. Yanling Xiao, et al., 2004, The Journal of Immunology, 172: 7432-41.