CD27 Antibody (Cytokine)

CD27 / TNFRSF7 Antibody Datasheet

CD27 / TNFRSF7 Antibody Product Information

• Immunogen: Recombinant Human CD27 / TNFRSF7 Protein (Catalog#10039-H03H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 036
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CD27 / TNFRSF7 (rh CD27 / TNFRSF7; Catalog#10039-H03H; Met 1 - Ile 192; NP_001233.1).

CD27 / TNFRSF7 Antibody Usage Guide

• Specificity: Human CD27 / TNFRSF7
• No cross-reactivity in ELISA with

  Mouse CD27

  Human TNFRSF8 / CD30

  Human 4-1BB

  Human CD40

  Human DR6

  Human FAS

  Human HVEM / TNFRSF14

  Human TNFRI

  Human TNFRII

  Human BAFF

  Human RELT

  Mouse HVEM

  Mouse FAS

  Rat FAS

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD27 in WB. Using a DAB detection system, the detection limit for Human CD27 is approximately 1 ng/lane under non-reducing. Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD27. The detection limit for Human CD27 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD27 / TNFRSF7 Antibody Background

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. The CD27 receptor transduces signals and subsequently leads to the activation of NF-kappaB and MAPK8/JNK, and during which, adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling processes. In addition, a proapoptotic protein named SIVA, is capable of binding the cytoplasmic tail of CD27 and exerts action in the process of apoptosis.

References

1. Borst. J. et al., 2005, Curr. Opin. Immunol. 17: 275-281.
2. Kobata. T. et al., 1995, Proc. Natl. Acad. Sci. 92: 11249-53.
3. Jacquot, S., 2000, Immunol Res. 21:23-30.
4. Arens, R. et al., 2004, J. Immunol. 173: 3901-3908.
5. Akiba, H. et al., 1998, J. Biol. Chem. 273: 13353 - 13358.
6. Fortin, A. et al., 2004, J. Biol. Chem. 279: 28706 – 28714.