Human CD25 / IL2Rα Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10165-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000417.1

cDNA Size:   819

Synonym:   IL2RA, CD25, IL2R, TCGFR, IDDM10

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens interleukin 2 receptor, alpha (IL2RA) DNA

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the 516 C/T point mutation not causing any amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂Oby briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGATTCAT ACCTGCTGAT GTGGGGACTG CTCACGTTCA TCATGGTGCC TGGCTGCCAG
61       GCAGAGCTCT GTGACGATGA CCCGCCAGAG ATCCCACACG CCACATTCAA AGCCATGGCC
121      TACAAGGAAG GAACCATGTT GAACTGTGAA TGCAAGAGAG GTTTCCGCAG AATAAAAAGC
181      GGGTCACTCT ATATGCTCTG TACAGGAAAC TCTAGCCACT CGTCCTGGGA CAACCAATGT
241      CAATGCACAA GCTCTGCCAC TCGGAACACA ACGAAACAAG TGACACCTCA ACCTGAAGAA
301      CAGAAAGAAA GGAAAACCAC AGAAATGCAA AGTCCAATGC AGCCAGTGGA CCAAGCGAGC
361      CTTCCAGGTC ACTGCAGGGA ACCTCCACCA TGGGAAAATG AAGCCACAGA GAGAATTTAT
421      CATTTCGTGG TGGGGCAGAT GGTTTATTAT CAGTGCGTCC AGGGATACAG GGCTCTACAC
481      AGAGGTCCTG CTGAGAGCGT CTGCAAAATG ACCCATGGGA AGACAAGGTG GACCCAGCCC
541      CAGCTCATAT GCACAGGTGA AATGGAGACC AGTCAGTTTC CAGGTGAAGA GAAGCCTCAG
601      GCAAGCCCCG AAGGCCGTCC TGAGAGTGAG ACTTCCTGCC TCGTCACAAC AACAGATTTT
661      CAAATACAGA CAGAAATGGC TGCAACCATG GAGACGTCCA TATTTACAAC AGAGTACCAG
721      GTAGCAGTGG CCGGCTGTGT TTTCCTGCTG ATCAGCGTCC TCCTCCTGAG TGGGCTCACC
781      TGGCAGCGGA GACAGAGGAA GAGTAGAAGA ACAATCTAG

Quality Control: cDNA sequencing results is available upon request.

IL2Rα / CD25 related areas, pathways, and other information

Cytokine