Human CD248 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13403-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD248 cDNA Clone Datasheet

CD248 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_020404.2
cDNA Size:	2274
Gene Synonym:	TEM1; CD164L1; CD248
Source:	Human

CD248 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens CD248 molecule, endosialin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1370 A/G resulting in the amino acid His substitution by Arg and 717 C/T not causing the amino acid variation.

CD248 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD248 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD248 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCTGC GCCTGTTGCT GGCCTGGGCG GCCGCAGGGC CCACACTGGG CCAGGACCCC
61       TGGGCTGCTG AGCCCCGTGC CGCCTGCGGC CCCAGCAGCT GCTACGCTCT CTTCCCACGG
121      CGCCGCACCT TCCTGGAGGC CTGGCGGGCC TGCCGCGAGC TGGGGGGCGA CCTGGCCACT
181      CCTCGGACCC CCGAGGAGGC CCAGCGTGTG GACAGCCTGG TGGGTGCGGG CCCAGCCAGC
241      CGGCTGCTGT GGATCGGGCT GCAGCGGCAG GCCCGGCAAT GCCAGCTGCA GCGCCCACTG
301      CGCGGCTTCA CGTGGACCAC AGGGGACCAG GACACGGCTT TCACCAACTG GGCCCAGCCA
361      GCCTCTGGAG GCCCCTGCCC GGCCCAGCGC TGTGTGGCCC TGGAGGCAAG TGGCGAGCAC
421      CGCTGGCTGG AGGGCTCGTG CACGCTGGCT GTCGACGGCT ACCTGTGCCA GTTTGGCTTC
481      GAGGGCGCCT GCCCGGCGCT GCAAGATGAG GCGGGCCAGG CCGGCCCAGC CGTGTATACC
541      ACGCCCTTCC ACCTGGTCTC CACAGAGTTT GAGTGGCTGC CCTTCGGCTC TGTGGCCGCT
601      GTGCAGTGCC AGGCTGGCAG GGGAGCCTCT CTGCTCTGCG TGAAGCAGCC TGAGGGAGGT
661      GTGGGCTGGT CACGGGCTGG GCCCCTGTGC CTGGGGACTG GCTGCAGCCC TGACAATGGG
721      GGCTGCGAAC ACGAATGTGT GGAGGAGGTG GATGGTCACG TGTCCTGCCG CTGCACTGAG
781      GGCTTCCGGC TGGCAGCAGA CGGGCGCAGT TGCGAGGACC CCTGTGCCCA GGCTCCGTGC
841      GAGCAGCAGT GTGAGCCCGG TGGGCCACAA GGCTACAGCT GCCACTGTCG CCTGGGTTTC
901      CGGCCAGCGG AGGATGATCC GCACCGCTGT GTGGACACAG ATGAGTGCCA GATTGCCGGT
961      GTGTGCCAGC AGATGTGTGT CAACTACGTT GGTGGCTTCG AGTGTTATTG TAGCGAGGGA
1021     CATGAGCTGG AGGCTGATGG CATCAGCTGC AGCCCTGCAG GGGCCATGGG TGCCCAGGCT
1081     TCCCAGGACC TCGGAGATGA GTTGCTGGAT GACGGGGAGG ATGAGGAAGA TGAAGACGAG
1141     GCCTGGAAGG CCTTCAACGG TGGCTGGACG GAGATGCCTG GGATCCTGTG GATGGAGCCT
1201     ACGCAGCCGC CTGACTTTGC CCTGGCCTAT AGACCGAGCT TCCCAGAGGA CAGAGAGCCA
1261     CAGATACCCT ACCCGGAGCC CACCTGGCCA CCCCCGCTCA GTGCCCCCAG GGTCCCCTAC
1321     CACTCCTCAG TGCTCTCCGT CACCCGGCCT GTGGTGGTCT CTGCCACGCG TCCCACACTG
1381     CCTTCTGCCC ACCAGCCTCC TGTGATCCCT GCCACACACC CAGCTTTGTC CCGTGACCAC
1441     CAGATCCCCG TGATCGCAGC CAACTATCCA GATCTGCCTT CTGCCTACCA ACCCGGTATT
1501     CTCTCTGTCT CTCATTCAGC ACAGCCTCCT GCCCACCAGC CCCCTATGAT CTCAACCAAA
1561     TATCCGGAGC TCTTCCCTGC CCACCAGTCC CCCATGTTTC CAGACACCCG GGTCGCTGGC
1621     ACCCAGACCA CCACTCATTT GCCTGGAATC CCACCTAACC ATGCCCCTCT GGTCACCACC
1681     CTCGGTGCCC AGCTACCCCC TCAAGCCCCA GATGCCCTTG TCCTCAGAAC CCAGGCCACC
1741     CAGCTTCCCA TTATCCCAAC TGCCCAGCCC TCTCTGACCA CCACCTCCAG GTCCCCTGTG
1801     TCTCCTGCCC ATCAAATCTC TGTGCCTGCT GCCACCCAGC CCGCAGCCCT CCCCACCCTC
1861     CTGCCCTCTC AGAGCCCCAC TAACCAGACC TCACCCATCA GCCCTACACA TCCCCATTCC
1921     AAAGCCCCCC AAATCCCAAG GGAAGATGGC CCCAGTCCCA AGTTGGCCCT GTGGCTGCCC
1981     TCACCAGCTC CCACAGCAGC CCCAACAGCC CTGGGGGAGG CTGGTCTTGC CGAGCACAGC
2041     CAGAGGGATG ACCGGTGGCT GCTGGTGGCA CTCCTGGTGC CAACGTGTGT CTTTTTGGTG
2101     GTCCTGCTTG CACTGGGCAT CGTGTACTGC ACCCGCTGTG GCCCCCATGC ACCCAACAAG
2161     CGCATCACTG ACTGCTATCG CTGGGTCATC CATGCTGGGA GCAAGAGCCC AACAGAACCC
2221     ATGCCCCCCA GGGGCAGCCT CACAGGGGTG CAGACCTGCA GAACCAGCGT GTGA

Quality Control:cDNA sequencing results is available upon request.