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Rat CD247 Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
RG80391-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD247 cDNA Clone Datasheet

 

CD247 cDNA Clone Product Information

Gene Bank Ref.ID: BC097933.1

cDNA Size:

495

Gene Synonym:

Cd3z; TCRzeta; Cd247

Source: Rat

CD247 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus Cd247 molecule DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD247 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD247 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD247 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG
61       GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC
121      ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT
181      GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACG AGCTCAACCT AGGGCGAAGA
241      GAGGAATATG ATGTTTTGGA CAAGAAGCGG CCTCGGGATC CGGAGATGGG GGGCAAACAG
301      CAGAGGAGGA GGAACCCCCA GGAAGGCGTG TACAATGCAC TGCAGAAAGA CAAGATGGCA
361      GAGGCCTACA GTGAGATTGG CATGAAAGGC GAGAGGCGGA GAGGCAAGGG GCACGACGGC
421      CTTTACCAGG GTCTCAGCAC TGCCACCAAG GACACCTATG ACGCCCTGCA TATGCAGACC
481      CTGCCCCCTC GCTAA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD247 (CD3-ZETA) related areas, pathways, and other information