CD23 / FCER2 Protein (His Tag)

CD23 / FCER2 Protein

CD23 / FCER2 Protein Product Information

• Synonym: CLEC-6, CLEC6, CLECSF8, MCL, MGC40078, MPCL
• Protein Construction: A DNA sequence encoding the human CD23 isoform 2 ( P06734-1 ) extracellular domain ( Asp 48 - Ser 321 ) was expressed, with a polyhistidine tag at the N-terminus
• Source: Human
• Expression Host: Human Cells

CD23 / FCER2 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human CD23 consists of 290 amino acids and has a calculated molecular mass of 33.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD23 is approximately 44 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD23 protein

CD23 / FCER2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD23 / FCER2 Protein Description

Low affinity immunoglobulin epsilon Fc receptor, also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23 and FCER2, is a secreted and single-pass type II membrane protein which is also exists as a soluble excreted form. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. Unlike many of the antibody receptors, CD23 / FCER2 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. In flow cytometry, CD23 / FCER2 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative). CD23 / FCER2 can also be demonstrated in germinal centre B-cells using immunohistochemistry, but it is not present in the resting cells of the surrounding mantle zone. CD23 / FCER2 has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

References

1. Kikutani H., et al., 1986, Cell 47:657-665.
2. Rose K., et al., 1992, Biochem. J. 286:819-824.
3. Padlan E.A., et al., 1993, Receptor 3:325-341.
4. Bajorath J., et al., 1996, Protein Sci. 5:240-247.
5. Barna G, et al., 2008, Hematol Oncol 26 (3): 167-70.