Rat DNAM-1 / CD226 Gene cDNA Clone / ORF Clone

CD226 cDNA Clone Datasheet

CD226 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001107370.1
• cDNA Size: 1042
• Gene Synonym: Cd226
• Source: Rat

CD226 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD226 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 1038 C insertion mutation.

CD226 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD226 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD226 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGAAGG CAGTCCAACC TCCTGAACCG TTTCCAGAGA TGGCTTATCT TACTTGGCTT
61       TTGGCTATTC TTCATGTGCA CAAAGCGCTG TGTGAAGAGA CATTGTGGGA CACAACTGTT
121      CGTCTTTCTG AGACTATGAC TTTGGAATGT GTATATCCAT TGACGGATAA CTTAACCCAG
181      GCGGAGTGGA CCAAGGACAC TGGCACAAAG AAAGTGAACA TAGCTGTTTA CCACCCTAAC
241      CATAATATGC ATATAGATCC TAACTACCTC CATAGAGTGC ACTTTCAAAA TGCAACAGTG
301      GGGTTCCGCA ACATGAGCCT TTCCTTTTAC AATGCCTCTG AAGCAGACAT CGGCATCTAC
361      ACCTGCTTGT TTCATGCTTT CCCACGTGGA TCTTGGGAAA AGAAGATAAA AGTTGTCCAG
421      TCAGATAGTT TTGAGACAAC AGCACCCTTG GATAGCTACC TGTCTGCAGA ACCTGGACAA
481      AATGTCACAC TCAGTTGCCA GCTTGAAAGG ACTTGGCTAG TGCAACTAGT CATATGGGAA
541      AAAGTCCAGC CCCATCAGGT AGACATCTTA GCTTCCTGTA ACCTATCTCA AGGGACAAGA
601      TACACTTCAA AGTACCTAAG ACAAACATGG AGCGACTGTA GCCAGGAGAG CATGAAGAGT
661      GCTCTCATTA TACCCTATGC CATGGCTGCT GACTCAGGAC TTTACAGATG CCGCTCTGAG
721      GCCAGTACGG GAACAAACAA AACCTGTGTC ATAAGGCTGA TCATAACTGA TGGTGGAACC
781      AATAACCACT TTATCCTTCT CATTGTCGGA GGGCTAGCCT CACTGCTACT TGTCATCCTA
841      ATTACCATCA TTATTATCTT ATTCAACAGG AAGAGAAGGA GACAGGTGAG AACTCCGCTT
901      AAAGAGCTCA GAGATAAACA GAGTAAGGTA GCCACCAACT GCAGAAGTCC AACTTCTCCC
961      ATTCAGACTA CAGATGATGA AAAAGAGGAC ATTTATGTTA ACTATCCAAC TTTCTCTCGG
1021     AGACCAAAAC CAAGATTCCT AA

 

Quality Control:cDNA sequencing results is available upon request.