CD221 / IGF1R (aa 954-1367) Protein (GST Tag)

Insulin-like growth factor 1 receptor Protein Datasheet

CD221 / IGF1R Protein Product Information

• Synonym: CD221, IGFIR, JTK13, MGC142170, MGC142172, MGC18216
• Protein Construction: A DNA sequence encoding the human IGF1R ( NP_000866.1) cytoplasmic domain (Met 954-Cys 1367) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

CD221 / IGF1R Protein QC Testing

• Purity: >88 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 20%glycerol, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD221 / IGF1R protein

CD221 / IGF1R Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD221 / IGF1R Protein Description

IGF1R (Insulin-like growth factor-1 receptor) is a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF1 receptor/IGF1R is a receptor tyrosine kinase (RTK) expressed in all cell types and tissues, and plays a critical role in transformation events during embryogenesis, development and pathogenesis processes. IGF1R become tyrosine phosphorylated and enzymatically activated either in response to IGF1 and 2 ligands or because of the activity of the Src tyrosine kinase, and subsequently initiates an intracellular signaling cascade (MAPK) that leads to gene expression changes and correspondent cellular responses. It is commonly overexpressed in most malignant tissues including breast, prostate, pancreas and affect cancer cell proliferation, adhesion, metastasis and survival by mediating the anti-apoptotic pathway. Accordingly, IGF1R is regarded as potential anti-tumor therapeutic target, and momoclonal antibodies are probably the most promising and specific therapeutic agents.

References

1. Tollefsen SE. et al., 1991, Biochemistry. 30: 48-54.
2. Peterson JE. et al., 1996, J Biol Chem. 271: 31562-71.
3. Peruzzi F. et al., 1999, Mol Cell Biol. 19: 7203-15.
4. Gross JM. et al., 2003, Cancer Metastasis Rev. 22: 327-36.
5. Sachdev D. et al., 2004, J Biol Chem. 279: 5017-24.