Cynomolgus monkey CD22 Gene cDNA Clone / ORF Clone

CD22 cDNA Clone Datasheet

CD22 cDNA Clone Product Information

• cDNA Size: 2541
• Gene Synonym: CD22
• Source: Cynomolgus monkey

CD22 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD22 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 34-39 deletion mutation and the point mutation 2347 A/G, resulting in the amino acid Lys substitution by Glu, 1596 C/T not causing the amino acid variation.
  Please check the sequence information before order.

CD22 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD22 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD22 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATCTCC TCGGCCCCTG GCTCCTGCTC CTGGAATACT TGGCTTTCTC TGACTCAAGT
61       AAATGGAATA TTGAGCACCC TGGAACCATC TACGCCTGGG AGGGGGCCTG CGTCTGGGTC
121      CCCTGCACCT ACAGAGTCCT AGATGGTGCC CTGGAAACCT TCATCCTGTT CCACAATCCT
181      GAGTATAACC AGAACATGTC GAAGTTCGAA GGGACCAGAC TCTATGAAAG CACAAAGGAT
241      GGGAAGGTTC CTTCAGGGCA GAAAAGGGTG CAATTCCTGG GGAACAAGAT TAACAACAAC
301      TGCACACTGA GTATCCACCC AGTGCACGTC AATGACAGTG GTCAGCTGGG GCTGAGGATG
361      GTGTCCAAGA CTGAGAAATG GATGGAACGA ATACACCTCA ATGTCTCCGA AAGGCCTTTT
421      CCACCTCGTA TCCAGCTCCC TCCAAAACTT CAAGAATCCC AGGAAGTCAC TCTGACCTGC
481      TTGCTGAATT TCTCCTGCTA TGGGTATCAG ATCCAATTGC AGTGGTTACT AGAGGGGGTT
541      CCAATGAGGC AGGCTGCTGT CACCTCGACC TCCTTGAGCA CCAAGTCTGT CTTCACCCGG
601      AGTGAGCTCA AGTTCTCGCC ACAGTGGAGT CACCATGGGA AGATCGTGAC CTGCGAGCTT
661      CACGATGTGG ACGGGAAGGT CCTCTCCGAA GACATGGTGC AGCTGAACGT GAAGCACACC
721      CCAAAGTTGA CGATCGAGGT CACTCCCAAT GAAACCATAG TGAGGAAGGG GGACTCTGTG
781      ACCATGACCT GCAAGGTCAA CAGCAGCAAC CCGGAGTACA CGACGGTATC CTGGCTCAAG
841      GATGGCATCC CGCTGAAGGA GCAGAATACG CTCATGCTAA CCCTGCACGA AGTGACCAAG
901      TCCCAGAGTG GGAGGTACTG CTGTAGGGTC TCCAATGACG TGGGCCCGGC AACGTCGGAA
961      AAAGTGTTCC TGCAAGTACA GTATGCCCCG GAACCTTCCA GGGTTCAGAT CTCCCAGTCA
1021     CCGGCTGTGG AGGGAAGTGA AGTCAACTTT CTTTGCATAT CACCAGCCAA TCCTCTTCCA
1081     ACAAATTACA CGTGGTACCA CAATGGCAAA GAAGTGCAGG GAAGGACAGA GAAGCAATTC
1141     CAGATCCAAA AGATCCTCCC CTGGCACGCT GGGACTTATT CCTGTGAGGC GGAAAATATT
1201     CTTGGTATTG GAGAGAGGGG CCCGGGAACT GAGCTGGATG TCCAGTATCC TCCCAAGAAG
1261     GTGACCATGG TGATTGAAAA CCCCACGCCG ATTCGAGAAG GAGACACGGT GACCCTTTCC
1321     TGTAACTACA GTTCCAGTAA CCCCATTGTT AACCACTATG AATGGAGACC CCGTGGCGCC
1381     TGGGAGGAGC CATCACTTGG GGTGCTGAAG ATCCAAAACA TTGGCTGGAA CAACACAGCC
1441     GTCGCCTGCG CAGCTTGTAA CAACTGGTGC TCGTGGGCCT CCCCTGTCAC CCTGAATGTC
1501     CTGTATGCCC CCCGAGGCGT GAGGGTCCGG AAAATCAAGC CCCTTTCTGA GATTCACTCT
1561     GGGAACTCGG TCAGCCTCCA ATGTGACTTT TCAAGCAGCC ACCCCAAAGA AGTCCAGTTC
1621     TTCTGGGAGA AAAATGGCAG CCTTCTGGGG AAAGAAAGCC AGCTGAATTT TGACTCCATC
1681     TCCCCAGAAG ATGCTGGGAG TTACAGCTGC TGGGTGAACA ACTCCATAGG ACAGACGGCG
1741     TCCAAGGCCT GGACACTCGA AGTGCTGTAT GCACCCAGGA GGCTGCGTGT GTCCATGAGC
1801     CAGGGGAACC AAGTGATGGA GGGGAAGACG GCAACCCTGA TCTGTGAGAG TGACGCCAAC
1861     CCTCCCGTCT ACAGCTACGC CTGGTTTGAC TGGAATAACC AAAGCCTTCC CTACTCCGGC
1921     CGGATGCTGA GATTGGAGCC GGTGAAGGTC CAGCACTCCG GTGCTTACTG GTGCCAGGGG
1981     ACCAACAGAG TGGGCAAGGG CCACTCGCCT CTCATCACCC TCACCGTCTA CTATAGCCCG
2041     CAGACCATTG GCAGGCGAGT GGCTGTGGGA CTCGGGTCCT GCCTGGCCAT CCTCATCCTG
2101     GCAATGTGTG GATTCAAGGT CCAGCGACGT TGGAAGAGGA CACAGAGCCA GCAGGGGCTT
2161     CAGGAGAATT CCAGTGGCCA GAGCTTCTTT GTGAGGAACA AAAAGGTTAG AAGGACCCCC
2221     CTCTCTGAAG GTCCCCACTC CCTGGGATGC TACAATCCGA TGATGGAAGA TGGCATTAGC
2281     TACGCCACCC TGCGCTTTCC CGAGACGAAC ACACCACGAA CTGGAGATGC AGAGACCTCC
2341     GAGCTGCAGA GACCTCCCCC AGACTGCGAT GACACAGTCA CTTATTCAGT GTTGCAGAAG
2401     CGTCAGGTGG GCGACTATGA GAACGTCATT CCAGATTTTC CAGAAGATGA GGGAATTCAT
2461     TACTCAGAGC TGATTCAGTT TGGGTTCGGG GAGCGGCCTC AGGCACAAGA AAATGTGGAC
2521     TATGTGATCG TCAAGCACTG A

 

Quality Control:cDNA sequencing results is available upon request.