Anti-CD22 Antibody

Cat: 11958-MM07
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Anti-CD22 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD22 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD22
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human CD22 protein (Catalog#11958-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD22 (rh CD22; Catalog#11958-H08H; P20273-1; Met 1-Arg 687). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #07
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD22 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD22 Antibody Alternative Names
Anti-SIGLEC-2 Antibody;Anti-SIGLEC2 Antibody
CD22 Background Information

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD22 molecule
References
  • Tedder TF, et al. (1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504.
  • Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
  • Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
  • Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
  • Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD22 Antibody Rabbit Polyclonal ELISA 81343-RP01
    Anti-CD22 Antibody Rabbit Monoclonal ELISA 81343-R072
    Anti-CD22 Antibody Rabbit Polyclonal ELISA,IHC-P 81343-T24
    Anti-CD22 Antibody Rabbit Monoclonal FCM 51177-R026
    Anti-CD22 Antibody (PE) Rabbit Monoclonal FCM 51177-R026-P
    Anti-CD22 Antibody Rabbit Monoclonal ELISA 51177-R064
    Anti-CD22 Antibody (PE) Rabbit Monoclonal FCM 51177-R060-P
    Anti-CD22 Antibody (FITC) Rabbit Monoclonal FCM 51177-R060-F
    Anti-CD22 Antibody (APC) Rabbit Monoclonal FCM 51177-R060-A
    Anti-CD22 Antibody Rabbit Monoclonal WB,IP 51177-R087
    Anti-CD22 Antibody Rabbit Monoclonal WB 51177-R078
    Anti-CD22 Antibody Rabbit Monoclonal FCM 51177-R060
    Anti-CD22 Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 11958-T26
    Anti-CD22 Antibody Mouse Monoclonal ELISA,IHC-P 11958-MM12
    Anti-CD22 Antibody Mouse Monoclonal FCM 11958-MM13
    Anti-CD22 Antibody Rabbit Polyclonal ELISA 11958-RP01
    Anti-CD22 Antibody Rabbit Monoclonal ELISA,IHC-P,ICC/IF,IF 11958-R340
    Anti-CD22 Antibody (PE) Mouse Monoclonal FCM 11958-MM13-P
    Anti-CD22 Antibody (FITC) Mouse Monoclonal FCM 11958-MM13-F
    Anti-CD22 Antibody (PerCP) Mouse Monoclonal FCM 11958-MM13-C
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