Anti-CD22 Antibody

Cat: 11958-MM07
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All CD22 Reagents
Anti-CD22 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD22 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD22
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Recombinant Human CD22 protein (Catalog#11958-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD22 (rh CD22; Catalog#11958-H08H; P20273-1; Met 1-Arg 687). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #07
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD22 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD22 Antibody Alternative Names
Anti-SIGLEC-2 Antibody;Anti-SIGLEC2 Antibody
CD22 Background Information

CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD22 molecule
  • Tedder TF, et al. (1997) CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504.
  • Tedder TF, et al. (2005) CD22: a multifunctional receptor that regulates B lymphocyte survival and signal transduction. Adv Immunol. 88: 1-50.
  • Fujimoto M, et al. (2007) B cell signaling and autoimmune diseases: CD19/CD22 loop as a B cell signaling device to regulate the balance of autoimmunity. J Dermatol Sci. 46(1): 1-9.
  • Walker JA, et al. (2008) CD22: an inhibitory enigma. Immunology. 123(3): 314-25.
  • Nitschke L. (2009) CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 230(1): 128-43.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD22 Antibody Rabbit Polyclonal ELISA 81343-RP01
    Anti-CD22 Antibody Rabbit Monoclonal ELISA 81343-R072
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    Anti-CD22 Antibody (PE) Rabbit Monoclonal FCM 51177-R026-P
    Anti-CD22 Antibody Rabbit Monoclonal ELISA 51177-R064
    Anti-CD22 Antibody (PE) Rabbit Monoclonal FCM 51177-R060-P
    Anti-CD22 Antibody (FITC) Rabbit Monoclonal FCM 51177-R060-F
    Anti-CD22 Antibody (APC) Rabbit Monoclonal FCM 51177-R060-A
    Anti-CD22 Antibody Rabbit Monoclonal WB,IP 51177-R087
    Anti-CD22 Antibody Rabbit Monoclonal WB 51177-R078
    Anti-CD22 Antibody Rabbit Monoclonal FCM 51177-R060
    Anti-CD22 Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 11958-T26
    Anti-CD22 Antibody Mouse Monoclonal ELISA,IHC-P 11958-MM12
    Anti-CD22 Antibody Mouse Monoclonal FCM 11958-MM13
    Anti-CD22 Antibody Rabbit Polyclonal ELISA 11958-RP01
    Anti-CD22 Antibody Rabbit Monoclonal ELISA,IHC-P,ICC/IF,IF 11958-R340
    Anti-CD22 Antibody (PE) Mouse Monoclonal FCM 11958-MM13-P
    Anti-CD22 Antibody (FITC) Mouse Monoclonal FCM 11958-MM13-F
    Anti-CD22 Antibody (PerCP) Mouse Monoclonal FCM 11958-MM13-C
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