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> ELISA > ELISA Pair Set > CD208 / DC-LAMP / LAMP3 ELISA Pair Set, Human ELISA Pair Set CD208 / DC-LAMP / LAMP3 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10527 |
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Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set
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Order or Inquire for CD208 / DC-LAMP / LAMP3 ELISA Pair Set |
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High affinity Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set |
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Detection limit - 31.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human CD208 / DC-LAMP / LAMP3 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family. DC-LAMP and CD68 share very similar predicted structure and are the only two members of this LAMP family with a single membrane-proximal (domain II) lamp-like domain, and a region considered as a mucin-like structure rich in serines and threonines in the N-terminal portion (domain I) . DC-LAMP is highly glycosylated: the observed mass of the mature glycoprotein on SDS-PAGE is around 70–90 kDa, whereas the predicted mass for the polypeptide core is 44 kDa. The presence of DC-LAMP in the MIIC in late mature DC indicates that this may perform an important function during the processing of exogenous antigens, and might also participate in the functional remodeling of the MIIC by facilitating the translocation of MHC class II molecules to the cell surface.Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
