LAMP3 Matched ELISA Antibody Pair Set,Human General Information
LAMP3 Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human LAMP3
1. Capture Antibody 1 mg/mL of rabbit anti-LAMP3 monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10527-R020)
2. Detection Antibody 0.5 mg/mL rabbit anti-LAMP3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10527-R002)
3. Standard Each vial contains 50 ng of recombinant LAMP3. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
This LAMP3 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human LAMP3 . It contains Human LAMP3 capture antibody, Human LAMP3 detector antibody
and a highly purified
recombinant Human LAMP3 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
LAMP3 Matched ELISA Antibody Pair Set,Human Images
LAMP3 Matched ELISA Antibody Pair Set,Human Alternative Names
CD208 Matched ELISA Antibody Pair Set, Human;DC LAMP Matched ELISA Antibody Pair Set, Human;DC-LAMP Matched ELISA Antibody Pair Set, Human;DCLAMP Matched ELISA Antibody Pair Set, Human;LAMP Matched ELISA Antibody Pair Set, Human;LAMP-3 Matched ELISA Antibody Pair Set, Human;TSC403 Matched ELISA Antibody Pair Set, Human
LAMP3 Background Information
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
lysosomal associated membrane protein 3
Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.