CD200RLa Antibody (Antigen Affinity Purified)

Cynomolgus CD200RLa Antibody ( Antigen Affinity Purified )

Cynomolgus CD200RLa Antibody Product Information

• Immunogen: Recombinant cynomolgus CD200RLa protein ( Catalog# 11576-C08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant cynomolgus CD200RLa ( rC CD200RLa ; Catalog# 11576-C08H ; Met 1 - Leu 240 ). CD200RLa specific IgG was purified by cynomolgus CD200RLa affinity chromatography

Cynomolgus CD200RLa Antibody Usage Guide

• Specificity: Cynomolgus CD200RLa
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect cynomolgus CD200rla in WB. Using a DAB detection system, the detection limit for cynomolgus CD200rla is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect cynomolgus CD200rla. The detection limit for cynomolgus CD200rla is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cynomolgus CD200RLa Antibody Background

Cynomolgus CD200 cell surface glycoprotein receptor-like a, also known as CD200RLa, is a member of the CD200R family. CD200 is a transmembrane protein delivering immunoregulatory signals after engagement of CD200R. A family of CD200Rs exist ( CD200R1, CD200R2, CD200R3, CD200R4 ) with different tissue expression and functional activity. CD200 (OX2) is a cell surface glycoprotein that interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. CD200 gave negligible binding to the activating receptors, CD200RLa, CD200RLb, and CD200RLc, by direct analysis at the protein level using recombinant monomeric and dimeric fusion proteins or to CD200RLa and CD200RLb when expressed at the cell surface.

References

1. Hatherley D. et al., 2005, J Immunol.Aug 175(4): 2469-74.
2. Jiang L. et al., 2009, Eur J Immunol. Apr. 39 (4):1167-75. 
3. Akkaya M. et al., 2010, Immunogenetics. Jan. 62(1):15-22. Epub 2009 Dec 5.