| Application | Dilution | Notes |
|---|---|---|
| WB | 1:500-1:1000 | |
| ELISA | 1:5000-1:10000 |
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CD200R1 rabbit polyclonal antibody at 1:500 dilution
Lane A: K562 Membrane Lysate Whole Cell Lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:37 kDa
Observed band size:37 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cell surface glycoprotein CD200 receptor 1 (CD200R1) is an isoform of CD200 receptors which is expressed on cells of the myeloid lineage. CD200R1 is a receptor for the OX-2 membrane glycoprotein. The receptor-substrate interaction may serve as a myeloid downregulatory signal.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
