Rat CD2 Gene cDNA Clone / ORF Clone

CD2 cDNA Clone Datasheet

CD2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012830.1
• cDNA Size: 1035
• Gene Synonym: CD2R, LFA2, OX34, LFA-2, OX-34, Cd2
• Source: Rat

CD2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus Cd2 molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 334 G/A resulting in the amino acid asp substitution by asn and 420 G/A，543 G/A not causing the amino acid variation.

CD2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGATGTA AATTCCTAGG GAGTTTCTTT CTGCTCTTCA GCCTGTCCAG CAAAGGAGCA
61       GACTGCAGAG ACAGTGGGAC CGTCTGGGGT GCCCTGGGTC ATGGCATCAA CCTGAACATC
121      CCTAACTTTC AAATGACTGA TGATATTGAT GAGGTGCGAT GGGAGAGGGG GAGCACCCTG
181      GTTGCCGAGT TTAAAAGGAA GATGAAGCCT TTTTTGAAAT CGGGAGCATT TGAGATCTTA
241      GCAAATGGAG ACTTGAAGAT AAAGAATCTG ACAAGAGATG ACAGTGGCAC CTATAATGTA
301      ACGGTATACA GCACAAATGG GACACGTATC CTGAACAAGG CACTGGACTT GAGGATTCTA
361      GAGATGGTCT CAAAGCCGAT GATCTACTGG GAGTGCAGCA ACGCAACCCT GACCTGTGAA
421      GTCTTGGAAG GAACAGATGT TGAACTAAAG CTGTACCAAG GAAAGGAGCA TCTCAGGAGC
481      CTCCGTCAGA AGACCATGAG TTACCAGTGG ACCAACCTGA GAGCACCGTT TAAGTGCAAG
541      GCAGTAAACA GGGTCAGCCA GGAGTCTGAG ATGGAAGTTG TCAACTGTCC AGAGAAAGGT
601      CTGCCCCTCT ATCTCATAGT GGGGGTCAGT GCAGGAGGAC TCCTCTTGGT GTTCTTTGGG
661      GCGCTGTTTA TTTTCTGTAT CTGCAAGAGG AAAAAACGGA ACAGGAGGAG AAAAGGTGAA
721      GAGCTGGAGA TAAAAGCTTC CAGAATGAGC ACTGTGGAAA GGGGACCCAA GCCACACTCA
781      ACCCAGGCCT CAGCACCAGC ATCACAGAAT CCAGTGGCTT CCCAAGCTCC TCCTCCACCT
841      GGCCATCATC TCCAGACACC TGGCCATCGT CCCCTGCCTC CAAGCCACCG TAACCGTGAG
901      CACCAGCCGA AGAAGAGACC TCCTCCATCA GGCACACAGG TTCACCAGCA GAAAGGCCCT
961      CCTTTGCCCA GACCCCGAGT TCAGCCAAAG CCTCCCTGTG GGAGTGGAGA TGTTTCGTTG
1021     CCACCCCCTA ATTAA

 

Quality Control:cDNA sequencing results is available upon request.