Rat CD2 Protein

Cd2 molecule Protein Datasheet

CD2 Protein Product Information

• Synonym: CD2
• Protein Construction: A DNA sequence encoding the rat CD2 (Q5I0M6) (Met1-Pro202) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD2 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 23
• Molecular Mass: The recombinant rat CD2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 421 amino acids and has a predicted molecular mass of 47.5 kDa. The apparent molecular mass of the protein is approximately 58-64 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD2 protein

CD2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD2 Protein Description

T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, CD2, and SRBC, is a single-pass type I  membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). Tremendous progress has been achieved in understanding the function of CD2, the mechanism of molecular recognition and protein folding. CD2 has been shown to set quantitative thresholds in T cell activation. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48 / BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. The expression of CD2 significantly increased with the severity of chronic HBV infection, which suggested that CD2 might contribute to the hepatocyte damage in chronic HBV infection.

References

1. Bai Y et al., 2002, Am J Transplant. 2 (7): 618-26.
2. Wilkins A. et al., 2003, Curr Protein Pept Sci. 4 (5): 367-73.
3. McNerney ME. et al., 2006, Curr Top Microbiol Immunol. 298 : 91-120.
4. Li J. et al., 2008, Cell Mol Immunol. 5 (1): 69-73.