CD19 Antibody

CD19 Antibody Datasheet

CD19 Antibody Product Information

• Immunogen: Recombinant Human CD19 Protein (Catalog #11880-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 05
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; Met 1-Lys 291; P15391). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD19 Antibody Usage Guide

• Specificity: Human CD19
• No cross-reactivity in ELISA with
  

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD19 in WB. Using a DAB detection system, the detection limit for Human CD19 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD19. The detection limit for Human CD19 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD19 Antibody Background

B-lymphocyte antigen CD19, also known as CD19, is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. It interacts with VAV. It interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. CD19 is an important pan B cell marker and co-stimulatory protein in humans and mice. CD19 is a hallmark differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 defines signaling thresholds for the Ag receptor as well as other cell surface receptors that regulate B lymphocyte selection, activation, and differentiation. Defects in CD19 are a cause of hypogammaglobulinemia.

References

1. Sato,S. et al., 1997, J Immunol. 158 (10): 4662-9.
2. Carter,RH. et al., 2002, Immunol Res. 26 (1-3): 45-54.
3. Ishikawa,H. et al., 2002, Leuk Lymphoma. 43 (3): 613-6.
4. Sun,J. et al., 2004, Mol Immunol. 41 (9): 929-38.