CD171 / N-CAML1 / L1CAM Antibody

CD171 / N-CAML1 / L1CAM Antibody Datasheet

CD171 / N-CAML1 / L1CAM Antibody Product Information

• Immunogen: Recombinant Human CD171 / N-CAML1 / L1CAM protein (Catalog#10140-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 01
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD171 / N-CAML1 / L1CAM (rh CD171 / N-CAML1 / L1CAM; Catalog#10140-H08H; NP_000416.1; Met 1-Glu 1120). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD171 / N-CAML1 / L1CAM Antibody Usage Guide

• Specificity: Human CD171 / N-CAML1 / L1CAM
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD171. The detection limit for Human CD171 is approximately 0.016 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD171 / N-CAML1 / L1CAM Antibody Background

L1 cell adhesion molecule (L1CAM), also designated as CD171, is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily, and has been implicated in a variety of processes integral to the development of the nervous system, including neuronal migration, neurite outgrowth, and axon fasciculation. It is primarily expressed on projection axons of the central nervous system and peripheral nervous system, as well as on a few nonneuronal cell types, such as Schwann cells and lymphocytes. The 1257 amino acid (aa) residue precursor protein contains an extracellular domain consisting of six Ig-like C2-type domains and five fibronectin-type III domains and highly conserved transmembrane and cytoplasmic domains. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan and intergrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs) and spastic paraplegia syndrome. In addition, L1CAM is also overexpressed in a variety of human carcinomas and is associated with bad prognosis.

References

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2. Wong, E.V. et al., 1995, Trends. Neurosci. 18: 168-172.
3. Wong, E.V. et al., 1996, J. Biol. Chem. 271: 18217-18233.
4. Bao, S. et al., 2008, Cancer. Res. 68: 6043-6048.
5. Gast, D. et al., 2008, Exp. Cell. Res. 314: 2411-2418.