CD16b / FCGR3B Protein (Native)

Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B Protein

CD16b / FCGR3B Protein Product Information

• Synonym: CD16, CD16b, FCG3, FCGR3, FCGR3B
• Protein Construction: A DNA sequence encoding the mature form of human CD16b ( O75015-1 ) ( Met 18 - Gly 193 ) was expressed and purified
• Source: Human
• Expression Host: E.coli

CD16b / FCGR3B Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of CD16b bound to Human IgG1 was 80nM.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CD16b comprises 176 amino acids and has a predicted molecular mass of 20 kDa. It migrates as an approximately 22 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris, 50mM Nacl, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD16b protein

CD16b / FCGR3B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16b / FCGR3B Protein Description

CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide-anchored form (CD16a) in human natural killer cells and macrophages and as a GPI-anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.

References

1. Nagarajan, S. et al., 1995,  J Biol Chem. 270 (43): 25762-70.
2. Middelhoven, P. J. et al., 1999, Biochem Biophys Res Commun. 255 (3): 568-74.
3. Williams, T. E. et al., 2000, Biophys J. 79 (4): 1867-75.
4. Fernandes, M. J.  et al., 2006,  Biochem J. 393 (Pt 1):  351-9.