CD16b / FCGR3B Protein (His Tag)

Fc Fragment of IgG, Low Affinity IIIb, Receptor Protein Datasheet

CD16b Protein Product Information

• CD16b Synonym: CD16, CD16b, FCG3, FCGR3,FCGR3B
• Protein Construction: A DNA sequence encoding the human CD16b (NP_000561.3) ( Met 1-Ser 200 ) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CD16b Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 17
• Molecular Mass: The recombinant human CD16b comprises 195 amino acids with a predicted molecular mass of 22.2 kDa. As a result of glycosylation, rh CD16b migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
• Formulation
• SDS-PAGE:

CD16b Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16b Protein Description

CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide-anchored form (CD16a) in human natural killer cells and macrophages and as a GPI-anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.

References

1. Nagarajan, S. et al., 1995, J Biol Chem. 270 (43): 25762-25770.
2. Middelhoven, P. J. et al., 1999, Biochem Biophys Res Commun. 255 (3):568-574.
3. Williams, T. E. et al., 2000, Biophys J. 79 (4): 1867-1875.
4. Fernandes, M. J. et al., 2006, Biochem J. 393 (Pt 1): 351-359.