CD16b / FCGR3B Protein (His Tag)

Fc fragment of IgG, low affinity IIIb, receptor Protein Datasheet

CD16b / FCGR3B Protein Product Information

• Synonym: CD16b, FCG3, FCGR3, IGFR3, FCGR3B
• Protein Construction: A DNA sequence encoding the human CD16b (NP_000561.3) ( Met 1-Ser 200 ) with a C-terminal polyhistidine tag was expressed.
• Source: Human
• Expression Host: CHO Cells

CD16b / FCGR3B Protein QC Testing

• Purity: > 99 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 17
• Molecular Mass: The recombinant human CD16b comprises 195 amino acids with a predicted molecular mass of 22.2 kDa. As a result of glycosylation, it migrates as an approximately 38-43 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD16b / FCGR3B protein

CD16b / FCGR3B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16b / FCGR3B Protein Description

CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide-anchored form (CD16a) in human natural killer cells and macrophages and as a GPI-anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.

References

1. Nagarajan S. et al., 1995, J Biol Chem. 270 (43): 25762-70.
2. Middelhoven P J. et al., 1999, Biochem Biophys Res Commun. 255 (3): 568-74.
3. Williams T E. et al., 2000, Biophys J. 79 (4): 1867-75.
4. Fernandes M J. et al., 2006, Biochem J. 393 (1): 351-9.