CD16b / FCGR3B Antibody

CD16b / FCGR3B / FcRIIIb Antibody Datasheet

CD16b / FCGR3B / FcRIIIb Antibody Product Information

• Immunogen: Recombinant human CD16b protein ( Catalog#11046-H08H )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 5A7F8C2
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD16b ( rh CD16b; Catalog#11046-H08H ; NP_000561.3 ; Met 1- Ser 200 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD16b / FCGR3B / FcRIIIb Antibody Usage Guide

• Specificity: Human CD16b / FCGR3B
• Has cross-reactivity in WB and ELISA with Human CD16a / FCGR3A
• No cross-reactivity in WB and ELISA with Human CD32a / FCGR2A , Human CD32b / FCGR2B and Human cell lysate (293 cell line)
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD16b in WB. Using a DAB detection system, the detection limit for human CD16b is approximately 20 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD16b / FCGR3B / FcRIIIb Antibody Background

CD16, the low affinity Fc gamma receptor III for IgG (FcgammaRIII), exists as a polypeptide-anchored form (CD16a) in human natural killer cells and macrophages and as a GPI-anchored form (CD16b) exclusively expressed on human neutrophils. CD16b is unique in that it is the only Fc receptor linked to the plasma membrane. The GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. CD16b constitutively partitions with both low- and high-density DRMs. Upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. It is indicated that CD16b associates with complement receptor 3 (CR3, Mac-1, CD11b/CD18) which can indirectly link CD16b to the actin cytoskeleton.

References

1. Nagarajan, S. et al., 1995, J Biol Chem. 270 (43): 25762-70.
2. Middelhoven, PJ. et al., 1999, Biochem Biophys Res Commun. 255 (3):568-74.
3. Williams, TE. et al., 2000, Biophys J. 79 (4): 1867-75.
4. Fernandes, M. J. et al., 2006, Biochem J. 393 (Pt 1): 351-9.