Human CD16a transcript variant 1 partial cDNA Clone / ORF Clone

FCGR3A cDNA Clone Datasheet

FCGR3A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000569.6
• cDNA Size: 702
• Gene Synonym: CD16; FCG3; CD16A; FCGR3; IGFR3; FCR-10; FCRIII; FCGRIII; FCRIIIA; FCGR3A
• Source: Human

FCGR3A cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens Fc fragment of IgG, low affinity IIIa, receptor (CD16a) DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

FCGR3A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCGR3A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

FCGR3A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGCAGC TGCTCCTCCC AACTGCTCTG CTACTTCTAG TTTCAGCTGG CATGCGGACT
61       GAAGATCTCC CAAAGGCTGT GGTGTTCCTG GAGCCTCAAT GGTACAGGGT GCTCGAGAAG
121      GACAGTGTGA CTCTGAAGTG CCAGGGAGCC TACTCCCCTG AGGACAATTC CACACAGTGG
181      TTTCACAATG AGAGCCTCAT CTCAAGCCAG GCCTCGAGCT ACTTCATTGA CGCTGCCACA
241      GTCGACGACA GTGGAGAGTA CAGGTGCCAG ACAAACCTCT CCACCCTCAG TGACCCGGTG
301      CAGCTAGAAG TCCATATCGG CTGGCTGTTG CTCCAGGCCC CTCGGTGGGT GTTCAAGGAG
361      GAAGACCCTA TTCACCTGAG GTGTCACAGC TGGAAGAACA CTGCTCTGCA TAAGGTCACA
421      TATTTACAGA ATGGCAAAGG CAGGAAGTAT TTTCATCATA ATTCTGACTT CTACATTCCA
481      AAAGCCACAC TCAAAGACAG CGGCTCCTAC TTCTGCAGGG GGCTTTTTGG GAGTAAAAAT
541      GTGTCTTCAG AGACTGTGAA CATCACCATC ACTCAAGGTT TGGCAGTGTC AACCATCTCA
601      TCATTCTTTC CACCTGGGTA CCAAGTCTCT TTCTGCTTGG TGATGGTACT CCTTTTTGCA
661      GTGGACACAG GACTATATTT CTCTGTGAAG ACAAACATTT GA

 

Quality Control:cDNA sequencing results is available upon request.