CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated

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CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated: Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA.
2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a(Cat:10389-H27H-B). The EC50 of Human CD16a(Cat:10389-H27H-B) is 2.4 - 5.7 μg/ml.
3. Labeling ratio of biotin to protein: 0.7-1.
4. Using the Octet RED System, the affinity constant (Kd) of CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated (Cat. 10389-H27H-B) bound to Human IgG1 was 10-6-10-8M.
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR3A158F/V in the reference.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gly 17
Molecule Mass
The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated: Images

CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated: Alternative Names

CD16 Protein, Human; CD16A Protein, Human; Fc gamma RIIIa Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCGRIII Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIA Protein, Human; IGFR3 Protein, Human; IMD20 Protein, Human

CD16a Background Information

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
References
  • David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
  • Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile
    Author
    Bruggeman, CW;Dekkers, G;Bentlage, AEH;Treffers, LW;Nagelkerke, SQ;Lissenberg-Thunnissen, S;Koeleman, CAM;Wuhrer, M;van den Berg, TK;Rispens, T;Vidarsson, G;Kuijpers, TW;
    Year
    2017
    Journal
    J. Immunol.
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