CD16a / FCGR3A Protein (His Tag)

Fc Fragment of IgG, Low Affinity IIIa, Receptor ( CD16a ) Protein

CD16a / FCGR3A Protein Product Information

• FCGR3A Synonym: RP11-5K23.1, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3
• Protein Construction: A DNA sequence encoding the extracellular domain ( Met 1 - Gln 208 ) of human CD16a ( P08637-1 ) precursor was expressed with a C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

CD16a / FCGR3A Protein QC Testing

• Purity: > 97% as determined by SDS-PAGE
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of CD16a bound to Human IgG1 was 180nM.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 17
• Molecular Mass: The secreted recombinant human CD16a consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD16a is approximately 45 - 50 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD16a protein

CD16a / FCGR3A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and usage. Avoid repeated freeze-thaw cycles
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD16a / FCGR3A Protein Description

Receptors for the Fc region of immunoglobin G (Fcγ R) are divided into three classes and CD16 (Fcγ RIII) is a multifunctional, low/intermediate affinity receptor. In human, CD16 is expressed as two distinct forms (CD16a and CD16b) encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages. CD16a has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. As an inflammatory mediator, CD16a receptor is involved in phagocytosis, secretion of enzymes, antibody-dependent cellular cytotoxicity (ADCC), mast cell degranulation, and clearance of immune complexes. Aberrant expression or mutations in this gene are implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

References

1. Peltz, G.A. et al., 1989, Proc. Natl. Acad. Sci. 86: 1013-1017.
2. Nagarajan, S. et al., 1995, J. Biol. Chem. 270: 25762-25770.
3. Kim, M-K. et al., 2003, Blood. 101: 4479-4484.
4. Nimmerjahn, F. et al., 2006, Immunity. 24:19-28.
5. Gessner, J.E. et al. 1995, Immunobiology. 193: 341-355.
6. Webster, N.L. et al., 2006, J. Leukoc. Biol. 79: 294 - 302.