Rat CD16a Gene cDNA Clone / ORF Clone

CD16a cDNA Clone Datasheet

CD16a cDNA Clone Product Information

• Gene Bank Ref.ID: NM_207603.1
• cDNA Size: 750
• Gene Synonym: Fcgr4; Fcgr3a
• Source: Rat

CD16a cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus Fc fragment of IgG, low affinity IIIa, receptor DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 83 A/T resulting in the amino acid Asn substitution by Ile.

CD16a cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD16a cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD16a cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGTACC TACTACTACC AACGGCACTG CTACTTACGG TTTCCTCTGG CGTTGGAGCT
61       GGTCTCCAAA AGGCTGTGGT GATCCTAGAC CCTGAATGGG TCAGGGTTCT CGAGGAAGAC
121      TGTGTGATCC TCAGGTGCCA GGGCACCTTC TCCCCCGAGG ACAATTCTAC CAAATGGTTC
181      CATAACAAAA GCCTCATCTC GCACCAGGAC GCCAACTATG TCATCCAAAG TGCCAGAGTT
241      AAGGACAGTG GAATGTACAG ATGCCAGACA GCCTTCTCCG CGCTCAGTGA CCCGGTGCAG
301      CTAGACGTCC ATGCAGACTG GCTATTGCTT CAGACCACTA AGCGGCTGTT CCAGGAGGGG
361      GACCCCATTC GTCTGAGATG CCATAGCTGG CGAAACACGC CTGTATTTAA GGTTACCTAT
421      TTACAGAATG GCAAAGGCAA GAAGTATTTC CATAGGAATT CTGAACTCAG TATTTCAAAA
481      GCTACGCACG CCGACAGTGG TTCCTACTTC TGCAGAGGGA TCATTGGACG CAACAACATA
541      TCTTCAGCAT CCTTGCAGAT AAGCATAGGA GATCCGACGT CTCCCTCCAG CTTTCTACCG
601      TGGCATCAAA TCACTTTCTG CCTGCTGATA GGACTCTTGT TTGCAATAGA CACAGTGCTG
661      TATTTCTCGG TGCAGAGGAG TCTTCAAAGT TCCGTGGCAG TCTATGAGGA ACCCAAACTT
721      CACTGGAGCA AGGAACCTCA GGACAAGTGA

 

Quality Control:cDNA sequencing results is available upon request.