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CD16a / FCGR3A Antibody, Mouse MAb

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Rat CD16a Antibody Product Information
Immunogen:Recombinant Rat CD16a / FCGR3A Protein (Catalog#80017-R08H)
Clone ID:4B1H6
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD16a / FCGR3A (rR CD16a / FCGR3A; Catalog #80017-R08H; Met 1-Pro 200; ABO70343.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Rat CD16a Antibody Usage Guide
Specificity:Rat CD16a / FCGR3A
No cross-reactivity with Human cell lysate (293 cell line) in ELISA
Application:ELISA

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat FCGR3A. The detection limit for Rat FCGR3A is approximately 0.31 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other CD16a Antibody Products
Reactivity: Human  
Application: IHC-P  
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10389-MM04-200
10389-MM04-100
10389-MM04-50
200 µg 
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    10389-MM02-50
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    Reactivity: Human  
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      10389-MM03-50
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      Reactivity: Human  
      Application: ELISA  
        10389-RP01-100
        10389-RP01-200
        10389-RP01-400
        100 µg 
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        Reactivity: Human  
        Application: ELISA  
          10389-RP02-50
          10389-RP02-100
          10389-RP02-200
          50 µg 
          100 µg 
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          Reactivity: Human  
          Application: ELISA  
            10389-MM05-100
            10389-MM05-50
            10389-MM05-200
            100 µg 
            50 µg 
            200 µg 
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            Reactivity: Mouse  
            Application: ELISA  IHC-P  
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            50086-R008-50
            50086-R008-100
            50 µg 
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            Reactivity: Rat  
            Application: ELISA  
              80017-RP01-400
              80017-RP01-200
              80017-RP01-100
               
              200 µg 
              100 µg 
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              Reactivity: Rat  
              Application: ELISA  WB  IP  
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              80017-RP02-50
              80017-RP02-100
              80017-RP02-200
              50 µg 
              100 µg 
              200 µg 
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              Reactivity: Rat  
              Application: ELISA  
                80017-R041-50
                80017-R041-100
                50 µg 
                100 µg 
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                CD16a/Fc gamma RIIIa Background

                The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.

                CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

                Rat CD16a/Fc gamma RIIIa References
                1. David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
                2. Edberg JC, et al. (2002) Genetic linkage and association of Fc gamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
                3. Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
                4. Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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                Catalog: 80017-MM06-50
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                All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"