Anti-CD16a Antibody

Cat: 80017-RP02
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Anti-CD16a Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD16a Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Rat
Specificity
Rat CD16a
Immunogen
Recombinant Rat CD16a / FCGR3A protein (Catalog#80017-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat CD16a / FCGR3A (rR CD16a / FCGR3A; Catalog#80017-R08H; Met 1-Pro 200; ABO70343.1). CD16a / FCGR3A specific IgG was purified by Rat CD16a / FCGR3A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD16a Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD16a Antibody (Rabbit Polyclonal antibody) Images
Rat CD16a/Fc gamma  RIIIa Immunoprecipitation(IP) 8265

rat FCGR3A was immunoprecipitated using:

Lane A:0.5 mg Raw264.7 Whole Cell Lysate

0.5 µL anti-rat FCGR3A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-rat FCGR3A rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 23 kDa

Rat CD16a/Fc gamma  RIIIa Western blot (WB) 13731

Anti-FCGR3 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:35 kDa

CD16a Background Information

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.

CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
References
  • David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD16a Antibody Rabbit Monoclonal ELISA 80017-R041
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    Anti-CD16a Antibody Rabbit Polyclonal ELISA 10389-RP02
    Anti-CD16a Antibody Mouse Monoclonal ELISA 10389-MM02
    Anti-CD16a Antibody Mouse Monoclonal IHC-P 10389-MM04
    Anti-CD16a Antibody Mouse Monoclonal ELISA 10389-MM03
    Anti-CD16a Antibody Rabbit Polyclonal ELISA,IHC-P 10389-T24
    Anti-CD16a Antibody Mouse Monoclonal ELISA 10389-MM05
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