Anti-CD16a Antibody


Anti-CD16a Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD16a Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD16a
Has cross-reactivity in ELISA with
Human CD16b
No cross-reactivity with Human CD32a, Human CD32b and Human cell lysate (293 cell line) in ELISA.
Recombinant Human CD16a protein (Catalog#10389-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16a (rhCD16a; Catalog#10389-H08H; NP_001121068.1; Met 1-Gln 208). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #4C9C3C4
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD16a Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD16a Antibody: Alternative Names

Anti-CD16 Antibody; Anti-CD16A Antibody; Anti-Fc gamma RIIIa Antibody; Anti-FCG3 Antibody; Anti-FCGR3 Antibody; Anti-FCGRIII Antibody; Anti-FCR-10 Antibody; Anti-FCRIII Antibody; Anti-FCRIIIA Antibody; Anti-IGFR3 Antibody; Anti-IMD20 Antibody

CD16a Background Information

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
  • David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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