Anti-CD16a Antibody

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Anti-CD16a Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-CD16a Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD16a
Has cross-reactivity in ELISA with
Human CD16b
No cross-reactivity with Human CD32a, Human CD32b and Human cell lysate (293 cell line) in ELISA.
Immunogen
Recombinant Human CD16a protein (Catalog#10389-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16a (rhCD16a; Catalog#10389-H08H; NP_001121068.1; Met 1-Gln 208). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #4C9C3C4
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD16a Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD16a Antibody: Alternative Names

Anti-CD16 Antibody; Anti-CD16A Antibody; Anti-Fc gamma RIIIa Antibody; Anti-FCG3 Antibody; Anti-FCGR3 Antibody; Anti-FCGRIII Antibody; Anti-FCR-10 Antibody; Anti-FCRIII Antibody; Anti-FCRIIIA Antibody; Anti-IGFR3 Antibody; Anti-IMD20 Antibody

CD16a Background Information

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Full Name
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
References
  • David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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