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CD16a / FCGR3A Antibody, Mouse MAb

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Human CD16a Antibody Product Information
Immunogen:Recombinant Human CD16a protein (Catalog#10389-H08H)
Clone ID:5B7F4D5
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16a (rhCD16a; Catalog#10389-H08H; NP_001121068.1; Met 1-Gln 208). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CD16a Antibody Usage Guide
Specificity:Human CD16a / FCGR3A / FcRIIIa
Has cross-reactivity in ELISA with
Human CD16b
Human CD32a
Human CD32b
No cross-reactivity with Human cell lysate (293 cell line) in ELISA.
Application:ELISA

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD16a. The detection limit for Human CD16a is approximately 0.625 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other CD16a Antibody Products
Reactivity: Human  
Application: IHC-P  
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10389-MM04-200
10389-MM04-100
10389-MM04-50
200 µg 
100 µg 
50 µg 
Add to Cart
Reactivity: Human  
Application: ELISA  
    10389-MM02-50
    10389-MM02-100
    50 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10389-RP01-100
      10389-RP01-200
      10389-RP01-400
      100 µg 
      200 µg 
       
      Add to Cart
      Reactivity: Human  
      Application: ELISA  
        10389-RP02-50
        10389-RP02-100
        10389-RP02-200
        50 µg 
        100 µg 
        200 µg 
        Add to Cart
        Reactivity: Human  
        Application: ELISA  
          10389-MM05-100
          10389-MM05-50
          10389-MM05-200
          100 µg 
          50 µg 
          200 µg 
          Add to Cart
          Reactivity: Mouse  
          Application: ELISA  IHC-P  
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          50086-R008-50
          50086-R008-100
          50 µg 
          100 µg 
          Add to Cart
          Reactivity: Rat  
          Application: ELISA  
            80017-RP01-400
            80017-RP01-200
            80017-RP01-100
             
            200 µg 
            100 µg 
            Add to Cart
            Reactivity: Rat  
            Application: ELISA  WB  IP  
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            80017-RP02-50
            80017-RP02-100
            80017-RP02-200
            50 µg 
            100 µg 
            200 µg 
            Add to Cart
            Reactivity: Rat  
            Application: ELISA  
              80017-MM06-50
              80017-MM06-100
              80017-MM06-200
              50 µg 
              100 µg 
              200 µg 
              Add to Cart
              Reactivity: Rat  
              Application: ELISA  
                80017-R041-50
                80017-R041-100
                50 µg 
                100 µg 
                Add to Cart
                CD16a/Fc gamma RIIIa Background

                The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.

                CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

                Human CD16a/Fc gamma RIIIa References
                1. David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
                2. Edberg JC, et al. (2002) Genetic linkage and association of Fc gamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
                3. Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
                4. Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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                Catalog: 10389-MM03-50
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                All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"