CD166 / ALCAM Antibody

CD166 / ALCAM Antibody Datasheet

CD166 / ALCAM Antibody Product Information

• Immunogen: Recombinant Human CD166 / ALCAM Protein (Catalog#10045-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 6E3C7
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD166 / ALCAM (rh CD166 / ALCAM; Catalog#10045-H08H; NP_001618.2; Met 1-Ala 526). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD166 / ALCAM Antibody Usage Guide

• Specificity: Human CD166 / ALCAM
• No cross-reactivity in ELISA with

  Human BCAM

  Human CD146

  Human CD171

  Human EpCAM

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ALCAM in WB. Using a DAB detection system, the detection limit for Human ALCAM is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ALCAM. The detection limit for Human ALCAM is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD166 / ALCAM Antibody Background

ALCAM (activated leukocyte cell adhesion molecule) is a type I membrane glycoprotein and a member of the immunoglobulin superfamily. It is also known as CD166, MEMD, SC-1/DM-GRASP in the chicken, and KG-CAM in the rat. ALCAM / CD166 has five extracellular immunoglobulin-like domains which facilitates heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. While expressed in a wide variety of tissues and cells, ALCAM is restricted to subsets of cells usually involved in dynamic growth and / or migration processes. ALCAM is highly associated not only with embryonic hematopoiesis but also vasculoangiogenesis. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other unidentified brain proteins, and thus is Involved in neurite extension by neurons via heterophilic and homophilic manners. ALCAM / CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- cells and B-cells to activated leukocytes. Further, ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines, suggesting a role in tumor migration.

References

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2. Aruffo, A. et al., 1997, Immunol. Today. 18: 498-504.
3. Degen, W. G. et al., 1998, Am. J. Pathol. 152: 805-813.
4. Van, K. L. et al., 2001, J. Biol. Chem. 276: 25783-257890.
5. Hirata, H. et al., 2006, Cells Tissues Organs.184 (3-4):172-180.
6. Jezierska, A. et al., 2006,Med Sci Monit.12 (7): BR245-256.