Rat CD164 / Endolyn Protein

CD164 molecule, sialomucin Protein Datasheet

CD164 / Endolyn Protein Product Information

• Synonym: CD164
• Protein Construction: A DNA sequence encoding the rat CD164 (Q9QX82) (Met1-Asp160) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD164 / Endolyn Protein QC Testing

• Purity: (84.8+14.7)% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 24
• Molecular Mass: The recombinant rat CD164/Fc is a disulfide-linked homodimer. The reduced monomer comprises 378 amino acids and has a predicted molecular mass of 41.3 kDa. The apparent molecular mass of the protein is approximately 90 and 35 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD164 / Endolyn protein

CD164 / Endolyn Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD164 / Endolyn Protein Description

Sialomucin core protein 24, also known as Endolyn, Multi-glycosylated core protein 24, MGC-24 and CD164, is a single-pass type I membrane and secreted protein which belongs to the CD164 family. Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play two key but opposing roles: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38 (lo/-) cell proliferation. CD164 functions as an adhesion receptor. It is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. CD164 promotes myogenesis by enhancing CXCR4-dependent cell motility. It positively regulates myoblast migration and promotes myoblast fusion into myotubes. CD164 may participate in the localization of prostate cancer cells to the marrow CD164 may play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cell.

References

1. Watt SM, et al., 1998, Blood. 92 (3): 849-66.
2. Zannettino A.C.W., et al., 1998, Blood 92:2613-2628.
3. Havens A.M., et al., 2006, BMC Cancer 6:195-195.
4. Forde S., et al., 2007, Blood 109:1825-1833.