Mouse CD163 Gene cDNA Clone / ORF Clone

CD163 cDNA Clone Datasheet

CD163 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053094.1
• cDNA Size: 3366
• Gene Synonym: CD163v2; CD163v3
• Source: Mouse

CD163 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus CD163 antigen DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 3366 A/T resulting in the amino acid Stop substitution by Tyr, and 1083 T/C, 2079 T/C not causing the amino acid variation.

CD163 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD163 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD163 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGTGGAC ACAGAATGGT TCTTCTTGGA GGTGCTGGAT CTCCTGGTTG TAAAAGGTTT
61       GTCCATCTAG GTTTCTTTGT TGTGGCTGTG AGCTCACTTC TCAGTGCCTC TGCTGTCACT
121      AACGCTCCTG GAGAAATGAA GAAGGAACTG AGACTGGCGG GTGGTGAAAA CAACTGTAGT
181      GGGAGAGTGG AACTTAAGAT CCATGACAAG TGGGGCACAG TGTGCAGTAA CGGCTGGAGC
241      ATGAATGAAG TGTCCGTGGT TTGCCAGCAG CTGGGATGCC CAACTTCTAT TAAAGCCCTT
301      GGATGGGCTA ACTCCAGCGC CGGCTCTGGA TATATCTGGA TGGACAAAGT TTCTTGTACA
361      GGGAATGAGT CAGCTCTTTG GGACTGCAAA CATGATGGGT GGGGAAAGCA TAACTGTACC
421      CATGAAAAAG ATGCTGGAGT GACCTGCTCA GATGGATCTA ATTTGGAGAT GAGACTGGTG
481      AACAGTGCGG GCCACCGATG CTTAGGAAGA GTAGAAATAA AGTTCCAGGG AAAGTGGGGG
541      ACGGTGTGTG ACGACAACTT CAGCAAAGAT CACGCTTCTG TGATTTGTAA ACAGCTTGGA
601      TGTGGAAGTG CCATTAGTTT CTCTGGCTCA GCTAAATTGG GAGCTGGTTC TGGACCAATC
661      TGGCTCGATG ACCTGGCATG CAATGGAAAT GAGTCAGCTC TCTGGGACTG CAAACACCGG
721      GGATGGGGCA AGCATAACTG TGACCATGCT GAGGATGTCG GTGTGATTTG CTTAGAGGGA
781      GCAGATCTGA GCCTGAGACT AGTGGATGGA GTGTCCAGAT GTTCAGGAAG ATTGGAAGTG
841      AGATTCCAAG GAGAATGGGG GACCGTGTGT GATGATAACT GGGATCTCCG GGATGCTTCT
901      GTGGTGTGCA AGCAACTGGG ATGTCCAACT GCCATCAGTG CCATTGGTCG AGTTAATGCC
961      AGTGAGGGAT CTGGACAGAT TTGGCTTGAC AACATTTCAT GCGAAGGACA TGAGGCAACT
1021     CTTTGGGAGT GTAAACACCA AGAGTGGGGA AAGCATTACT GTCATCATAG AGAAGACGCT
1081     GGCGTGACAT GTTCTGATGG AGCAGATCTG GAACTTAGAC TTGTAGGTGG AGGCAGTCGC
1141     TGTGCTGGCA TTGTGGAGGT GGAGATTCAG AAGCTGACTG GGAAGATGTG TAGCCGAGGC
1201     TGGACACTGG CAGATGCGGA TGTGGTTTGC AGACAGCTTG GATGTGGATC TGCGCTTCAA
1261     ACCCAGGCTA AGATCTACTC TAAAACTGGG GCAACAAATA CGTGGCTCTT TCCTGGATCT
1321     TGTAATGGAA ATGAAACTAC TTTTTGGCAA TGCAAAAACT GGCAGTGGGG CGGCCTTTCC
1381     TGTGATAATT TCGAAGAAGC CAAAGTTACC TGCTCAGGCC ACAGGGAACC CAGACTGGTT
1441     GGAGGAGAAA TCCCATGCTC TGGTCGTGTG GAAGTGAAAC ACGGAGACGT GTGGGGCTCC
1501     GTCTGTGATT TTGACTTGTC TCTGGAAGCT GCCAGTGTGG TGTGCAGGGA ATTACAATGT
1561     GGAACAGTCG TCTCTATCCT AGGGGGAGCA CATTTTGGAG AAGGAAGTGG ACAGATCTGG
1621     GGTGAAGAAT TCCAGTGTAG TGGGGATGAG TCCCATCTTT CACTATGCTC AGTGGCGCCC
1681     CCGCTAGACA GAACTTGTAC CCACAGCAGG GATGTCAGCG TAGTCTGCTC ACGATACATA
1741     GATATTCGTC TGGCAGGCGG CGAGTCCTCC TGTGAGGGAA GAGTGGAGCT CAAGACACTC
1801     GGAGCCTGGG GTCCCCTCTG CAGTTCTCAT TGGGACATGG AAGATGCTCA TGTCTTATGT
1861     CAGCAGCTGA AGTGTGGGGT TGCCCAATCT ATTCCAGAAG GAGCACATTT TGGGAAAGGA
1921     GCTGGTCAGG TCTGGAGTCA CATGTTCCAC TGCACTGGAA CTGAGGAACA TATAGGAGAT
1981     TGCCTCATGA CTGCTCTGGG TGCGCCGACG TGTTCCGAAG GACAGGTGGC CTCTGTCATC
2041     TGCTCAGGAA ACCAATCCCA GACACTATTG CCATGTAGCT CATTGTCTCC AGTCCAAACA
2101     ACAAGCTCTA CAATTCCAAA GGAGAGTGAA GTTCCCTGCA TAGCAAGTGG CCAGCTTCGC
2161     TTGGTAGGTG GAGGTGGTCG CTGCGCTGGA AGAGTGGAGG TCTACCACGA GGGCTCTTGG
2221     GGCACCGTCT GTGATGACAA TTGGGATATG ACTGATGCCA ATGTGGTGTG CAAGCAGCTG
2281     GACTGTGGCG TGGCAATTAA CGCCACTGGC TCTGCTTACT TCGGGGAAGG AGCAGGAGCT
2341     ATCTGGCTAG ACGAAGTCAT CTGCACTGGG AAAGAGTCTC ATATTTGGCA GTGCCATTCA
2401     CATGGCTGGG GACGCCATAA CTGCAGGCAC AAAGAAGATG CAGGTGTTAT CTGCTCCGAG
2461     TTCATGTCTC TGAGGCTGAC CAACGAAGCC CACAAAGAAA ACTGCACAGG TCGCCTTGAA
2521     GTGTTTTACA ATGGTACATG GGGCAGTATT GGCAGTAGCA ATATGTCTCC AACCACTGTG
2581     GGGGTGGTGT GCCGTCAGCT GGGCTGTGCA GACAACGGGA CTGTGAAACC CATACCTTCA
2641     GACAAGACAC CATCCAGGCC CATGTGGGTA GATCGTGTGC AGTGTCCAAA AGGAGTTGAC
2701     ACTTTGTGGC AGTGCCCCTC GTCACCTTGG AAACAGAGAC AGGCCAGCCC CTCCTCCCAG
2761     GAGTCCTGGA TCATCTGTGA CAACAAAATA AGACTCCAGG AAGGGCATAC AGACTGTTCT
2821     GGACGTGTGG AGATCTGGCA CAAAGGTTCC TGGGGAACAG TGTGTGATGA CTCCTGGGAT
2881     CTTAATGATG CTAAGGTTGT ATGTAAGCAG TTGGGCTGTG GCCAAGCTGT GAAGGCACTA
2941     AAAGAAGCAG CATTTGGTCC AGGAACTGGG CCCATATGGC TCAATGAAAT TAAGTGTAGA
3001     GGGAATGAGT CTTCCCTGTG GGATTGTCCT GCCAAACCGT GGAGTCACAG CGACTGTGGG
3061     CACAAAGAAG ATGCTTCCAT CCAGTGCCTC CCAAAAATGA CTTCAGAATC ACATCATGGC
3121     ACAGGTCACC CCACCCTCAC GGCACTCTTG GTTTGTGGAG CCATTCTATT GGTCCTCCTC
3181     ATTGTCTTCC TCCTGTGGAC TCTGAAGCGA CGACAGATTC AGCGACTTAC AGTTTCCTCA
3241     AGAGGAGAGG TCTTGATACA TCAAGTTCAG TACCAAGAGA TGGATTCAAA GGCGGATGAT
3301     CTGGACTTGC TGAAATCCTC GGGGGTCATT CAGAGGCACA CTGAGAAGGA AAATGATAAT
3361     TTATAT

 

Quality Control:cDNA sequencing results is available upon request.