CD16 / FCGR3 Antibody (Antigen Affinity Purified)

CD16 / FCGR3 Antibody ( Antigen Affinity Purified ) Datasheet

CD16 / FCGR3 Antibody Product Information

• Immunogen: Recombinant Cynomolgus CD16 / FCGR3 protein (Catalog#90013-C08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Cynomolgus CD16 / FCGR3 (rC CD16 / FCGR3; Catalog#90013-C08H; ABD83657.1; Met 1-Gly 206). CD16 / FCGR3 specific IgG was purified by Cynomolgus CD16 / FCGR3 affinity chromatography.

CD16 / FCGR3 Antibody Usage Guide

• Specificity: Cynomolgus CD16 / FCGR3
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus FCGR3 in WB. Using a DAB detection system, the detection limit for Cynomolgus FCGR3 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus FCGR3. The detection limit for Cynomolgus FCGR3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD16 / FCGR3 Antibody Background

Low affinity immunoglobulin gamma Fc region receptor III, also known as IgG Fc receptor III, Fc-gamma RIII, FcRIII, FCGR3 and CD16, is a single-pass type I membrane protein. It is expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. Mouse CD16 / FCGR3 is a receptor for the Fc region of complexed immunoglobulins gamma. It is a low affinity receptor. It contains twoIg-like C2-type (immunoglobulin-like) domains. In human, CD16 is expressed as two distinct forms (CD16a and CD16b) encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages.

References

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