Mouse CD16-2 / FCGR4 Protein (His Tag)

Fc receptor, IgG, low affinity IV Protein Datasheet

FcgR4 / CD16-2 Protein Product Information

• FcgR4 Synonym: 4833442P21Rik, CD16-2, FcgRIV, FcgammaRIV, Fcgr3a, Fcrl3, Fcgr4
• Protein Construction: The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

FcgR4 / CD16-2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The recombinant mouse FCGR4 consists of 194 amino acids and has a predicted molecular mass of 22.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE:

FcgR4 / CD16-2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FcgR4 / CD16-2 Protein Description

Receptors for the Fc region of IgG (Fc γ Rs) constitute a subfamily of the Ig superfamily , and function in the activation or inhibition of immune responses. Based on close relationships in their extracellular domains, the Fc γ R have been divided into three classes designated as Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Human CD16 is encoded by two genes: Fc γ RIIIA and Fc γ RIIIB, and the newly described mouse FCGR4, also referred to as CD16-2, has been proposed to be the mouse homolog of human Fc γ RIIIA because of high sequence homology (60 % aa identity). FCGR4 is expressed in peripheral blood leukocytes, spleen, thymus, colon and intestine, and serves as an activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory protein. Although mouse FCGR4 functioned as a low-affinity IgE receptor for all IgE allotypes, ligation of FCGR4 by antigen-IgE(b) immune complexes promotes macrophage-mediated phagocytosis, presentation of antigen to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions.

References

1. Mancardi D.A. et al., 2008, J Clin Invest. 118:3738-3750.
2. Minard-Colin V. et al., 2008, Blood. 112:1205-1213.
3. Baudino L. et al., 2008, J Immunol. 180:1948-1953.
4. Hirano M. et al., 2007, Nat Immunol. 8:762-771.
5. Jakus Z. et al., 2008, J Immunol. 180:618-629.