Mouse CD16-2 / FCGFR4 Gene cDNA Clone / ORF Clone

FCGR4 cDNA Clone Datasheet

FCGR4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_144559.1
• cDNA Size: 750
• Gene Synonym: Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik; Fcgr4
• Source: Mouse

FCGR4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Fc receptor, IgG, low affinity IV DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

FCGR4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCGR4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

FCGR4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGCAGC TACTACTACC AACAGCTCTG GTACTTACAG CTTTCTCTGG CATTCAAGCT
61       GGTCTCCAAA AGGCTGTGGT GAACCTAGAC CCCAAGTGGG TCAGGGTGCT TGAGGAAGAC
121      AGCGTGACCC TCAGATGCCA AGGCACTTTC TCCCCCGAGG ACAATTCTAT CAAGTGGTTC
181      CATAACGAAA GCCTCATCCC ACACCAGGAT GCCAACTATG TCATCCAAAG TGCCAGAGTT
241      AAGGACAGTG GAATGTACAG GTGCCAGACA GCCCTCTCCA CGATCAGTGA CCCAGTGCAA
301      CTAGAGGTCC ATATGGGCTG GCTATTGCTT CAGACCACTA AGTGGCTGTT CCAGGAGGGG
361      GACCCCATTC ATCTGAGATG CCACAGTTGG CAAAACAGAC CTGTACGGAA GGTCACCTAT
421      TTACAGAACG GCAAAGGCAA GAAGTATTTC CATGAAAATT CTGAATTACT CATTCCAAAA
481      GCTACACACA ATGACAGTGG CTCCTACTTC TGCAGAGGGC TCATTGGACA CAACAACAAA
541      TCTTCAGCAT CCTTTCGTAT AAGCCTAGGC GATCCAGGGT CTCCATCCAT GTTTCCACCG
601      TGGCATCAAA TCACATTCTG CCTGCTGATA GGACTCTTGT TTGCAATAGA CACAGTGCTG
661      TATTTCTCTG TGCGGAGGGG TCTTCAAAGT CCTGTGGCTG ACTATGAGGA ACCCAAGATT
721      CAATGGAGCA AGGAACCTCA GGACAAGTGA

 

Quality Control:cDNA sequencing results is available upon request.