Anti-FCGR4 Antibody

Cat: 50036-R012
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Anti-FCGR4 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-FCGR4 Antibody
Validated applications
ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Mouse
Specificity
Mouse FCGR4
Has cross-reactivity in ELISA with Human CD64 / FCGR1A
No cross-reactivity in ELISA with Human CD16a / FCGR3A / FcRIIIa Human CD16b / FCGR3B / FcRIIIb Human CD32a / FCGR2A Human CD32b / FCGR2B Mouse CD16 / FCGR3
Immunogen
Recombinant Mouse FCGR4 protein (Catalog#50036-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR4 (rM FCGR4; Catalog#50036-M08H; NP_653142.2; Met 1-Gln 203).
Source
Monoclonal Rabbit IgG Clone #012
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-FCGR4 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-FCGR4 Antibody (Rabbit Monoclonal antibody) Images
Mouse CD16-2/FCGR4 Immunofluorescence(IF) 2801
Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Mouse CD16-2/FCGR4 Flow Cytometry (FC) 5363
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
Anti-FCGR4 Antibody Alternative Names
Anti-4833442P21Rik Antibody;Anti-CD16-2 Antibody;Anti-FcgammaRIV Antibody;Anti-Fcgr3a Antibody;Anti-FcgRIV Antibody;Anti-Fcrl3 Antibody
FCGR4 Background Information
Full Name
Fc receptor, IgG, low affinity IV
Product Description Host Clonality Application Catalog# (PDF)
Anti-FCGR4 Antibody (PE) Rabbit Monoclonal FCM 50036-R012-P
Anti-FCGR4 Antibody (APC) Rabbit Monoclonal FCM 50036-R012-A
Anti-FCGR4 Antibody Rabbit Monoclonal ELISA,IHC-P 50036-R011
Anti-FCGR4 Antibody Rabbit Polyclonal ELISA 50036-RP01
Anti-FCGR4 Antibody (PerCP) Rabbit Monoclonal FCM 50036-R012-C
Anti-FCGR4 Antibody (FITC) Rabbit Monoclonal FCM 50036-R012-F
Anti-FCGR4 Antibody Rabbit Polyclonal ELISA 50036-RP02
info info
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