CD16-2 / FCGR4 Antibody

Mouse CD16-2 / FCGR4 Antibody

CD16-2 / FCGR4 Antibody Product Information

• Immunogen: Recombinant mouse FCGR4 protein ( Catalog#50036-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 12
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse FCGR4 ( rM FCGR4 ; Catalog#50036-M08H ; NP_653142.2 ; Met 1 -Gln 203 ).

CD16-2 / FCGR4 Antibody Usage Guide

• Specificity: Mouse CD16-2 / FCGR4
• Has cross-reactivity in ELISA with

  Human CD64 / FCGR1A

  No cross-reactivity in ELISA with

  Human CD16a / FCGR3A / FcRIIIa

  Human CD16b / FCGR3B / FcRIIIb

  Human CD32a / FCGR2A

  Human CD32b / FCGR2B

  Mouse CD16 / FCGR3

• Flow Cytometry: Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
  

  Flow cytometric analysis of FCGR4 expression on Raw264.7 cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse FCGR4 in WB.Using a DAB detection system, the detection limit for mouse FCGR4 is approximately 0.125 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse FCGR4. The detection limit for mouse FCGR4 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD16-2 / FCGR4 Antibody Background

Receptors for the Fc region of IgG (Fc γ Rs) constitute a subfamily of the Ig superfamily , and function in the activation or inhibition of immune responses. Based on close relationships in their extracellular domains, the Fc γ R have been divided into three classes designated as Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). Human CD16 is encoded by two genes: Fc γ RIIIA and Fc γ RIIIB, and the newly described mouse FCGR4, also referred to as CD16-2, has been proposed to be the mouse homolog of human Fc γ RIIIA because of high sequence homology (60% aa identity). FCGR4 is expressed in peripheral blood leukocytes, spleen, thymus, colon and intestine, and serves as an activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory protein. Although mouse FCGR4 functioned as a low-affinity IgE receptor for all IgE allotypes, ligation of FCGR4 by antigen-IgE(b) immune complexes promotes macrophage-mediated phagocytosis, presentation of antigen to T cells, production of proinflammatory cytokines and the late phase of cutaneous allergic reactions.

References

1. Mancardi D.A. et al., 2008, J Clin Invest. 118: 3738-3750.
2. Minard-Colin V. et al., 2008, Blood. 112: 1205-1213.
3. Baudino L. et al., 2008, J Immunol. 180: 1948-1953.
4. Hirano M. et al., 2007, Nat Immunol. 8: 762-771.
5. Jakus Z. et al., 2008, J Immunol. 180: 618-629.