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CD16-2 / FCGR4 Antibody, Rabbit MAb

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Mouse FCGR4 Antibody Product Information
Immunogen:Recombinant Mouse FCGR4 protein (Catalog#50036-M08H)
Clone ID:012
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR4 (rM FCGR4; Catalog#50036-M08H; NP_653142.2; Met 1-Gln 203).
Mouse FCGR4 Antibody Usage Guide
Specificity:Mouse CD16-2 / FCGR4
Has cross-reactivity in ELISA with
Human CD64 / FCGR1A
No cross-reactivity in ELISA with
Human CD16a / FCGR3A / FcRIIIa
Human CD16b / FCGR3B / FcRIIIb
Human CD32a / FCGR2A
Human CD32b / FCGR2B
Mouse CD16 / FCGR3
Application:ELISA, ICC/IF, FCM, IF

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FCGR4. The detection limit for Mouse FCGR4 is 0.00245 ng/well.

FCM: 0.5-2 μg/Test

ICC/IF: 5-20 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Mouse FCGR4 Antibody IF Application Image
CD16-2 / FCGR4 Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Mouse FcGR4 in RAW264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse FcGR4 monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Mouse FCGR4 Antibody FC Application Image
CD16-2 / FCGR4 Antibody, Rabbit MAb, Flow cytometric
[Click to enlarge image]
Caption:
Flow cytometric analysis of FCGR4 expression on Raw264.7 cells. Mouse leukaemic monocyte macrophage cell line RAW 264.7 were stained with anti-mouse FCGR4 (50036-R012, 1 μg/test), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
Other FCGR4 Antibody Products
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Catalog: 50036-R012-50
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50 µg
100 µg
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"